Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 4.4.1.5 extracted from

  • He, M.M.; Clugston, S.L.; Honek, J.F.; Matthews, B.W.
    Determination of the Structure of Escherichia coli Glyoxalase I Suggests a Structural Basis for Differential Metal Activation (2000), Biochemistry, 39, 8719-8727.
    View publication on PubMed

Crystallization (Commentary)

Crystallization (Comment) Organism
crystallization of apo GlxI and GlxI complexed with Ni2+ Co2+, Cd2+, Zn2+, and seleno-L-methionine-Ni2+ by vapor diffusion in hanging drops, 0.005 ml protein solution at 12-37 mg/ml is mixed with an equal volume of well solution containing 5-10% polyethylene glycol 1000 and 5-10% polyethylene glycol 8000, crystals diffract to 1.5-2.5 A, Zn2+-GlxI complex has trigonal bipyramidal instead of octhedral coordination with Ni2+, Co2+ and Cd2+ and is inactive Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Cd2+ reduced activity compared to Ni2+ Escherichia coli
Co2+ reduced activity compared to Ni2+ Escherichia coli
Mn2+ reduced activity compared to Ni2+ Escherichia coli
additional information no activity in the presence of Zn2+ Escherichia coli
Ni2+ maximal activity in the presence of Ni2+ Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
glutathione-methylglyoxal hemithioacetal Escherichia coli glutathione-methylglyoxal hemithioacetal is formed non-enzymatically from methylglyoxal and glutathione (R)-S-lactoylglutathione
-
?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glutathione-methylglyoxal hemithioacetal glutathione-methylglyoxal hemithioacetal is formed non-enzymatically from methylglyoxal and glutathione Escherichia coli (R)-S-lactoylglutathione
-
?

Synonyms

Synonyms Comment Organism
GLXI
-
Escherichia coli
glyoxalase I
-
Escherichia coli