Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Onchocerca volvulus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
dichlorophen | 0.048 mM, 50% inhibition | Onchocerca volvulus | |
formononetin 7-O-glucoside | 0.074 mM, 50% inhibition | Onchocerca volvulus | |
oxibendazol | 0.11 mM, 50% inhibition | Onchocerca volvulus | |
purpurogallin | 0.015 mM, 50% inhibition | Onchocerca volvulus | |
S-hexylglutathione | 0.11 mM, 50% inhibition | Onchocerca volvulus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.31 | - |
Phenylglyoxal | - |
Onchocerca volvulus | |
0.34 | - |
methylglyoxal | - |
Onchocerca volvulus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
24000 | - |
2 * 24000, SDS-PAGE | Onchocerca volvulus |
45000 | - |
gel filtration | Onchocerca volvulus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
glutathione-methylglyoxal hemithioacetal | Onchocerca volvulus | glutathione-methylglyoxal hemithioacetal is formed non-enzymatically from methylglyoxal and glutathione | (R)-S-lactoylglutathione | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Onchocerca volvulus | - |
causative agent of onchocerciasis, GloI expression is induced by oxidative stress | - |
Purification (Comment) | Organism |
---|---|
recombinant glyoxalase I | Onchocerca volvulus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
105 | - |
towards phenylglyoxal | Onchocerca volvulus |
284 | - |
towards methylglyoxal | Onchocerca volvulus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glutathione + methylglyoxal | - |
Onchocerca volvulus | S-D-lactoylglutathione | - |
? | |
glutathione + phenylglyoxal | - |
Onchocerca volvulus | S-mandeloylglutathione | - |
? | |
glutathione-methylglyoxal hemithioacetal | glutathione-methylglyoxal hemithioacetal is formed non-enzymatically from methylglyoxal and glutathione | Onchocerca volvulus | (R)-S-lactoylglutathione | - |
? | |
glutathione-phenylglyoxal hemithioacetal | glutathione-phenylglyoxal hemithioacetal is formed non-enzymatically from phenylglyoxal and glutathione | Onchocerca volvulus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 24000, SDS-PAGE | Onchocerca volvulus |
Synonyms | Comment | Organism |
---|---|---|
GloI | - |
Onchocerca volvulus |
glyoxalase I | - |
Onchocerca volvulus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.53 | - |
Phenylglyoxal | - |
Onchocerca volvulus | |
11.4 | - |
methylglyoxal | - |
Onchocerca volvulus | |
81.7 | - |
Phenylglyoxal | - |
Onchocerca volvulus |