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Literature summary for 4.4.1.5 extracted from

  • Paulus, C.; Köllner, B.; Jacobsen, H.J.
    Physiological and biochemical characterization of glyoxalase I, a general marker for cell proliferation, from a soybean cell suspension (1993), Planta, 189, 561-566.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.46
-
methylglyoxal/glutathione hemithioacetal
-
Glycine max

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
26000
-
1 * 26000 + 1 * 29000, SDS-PAGE Glycine max
29000
-
1 * 26000 + 1 * 29000, SDS-PAGE Glycine max
60000
-
gel filtration Glycine max

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Glycine max the enzyme is associated with cell proliferation, the activity is modulated during the proliferation cycle with a maximal activity between day 2 and day 4 of culture growth ?
-
?

Organism

Organism UniProt Comment Textmining
Glycine max
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Glycine max

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Glycine max

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glutathione + methylglyoxal the substrat is the hemithioacetal of methylglyoxal and glutathione Glycine max S-lactoylglutathione
-
?
glutathione-methylglyoxal hemithioacetal
-
Glycine max (R)-S-lactoylglutathione
-
?
additional information the enzyme is associated with cell proliferation, the activity is modulated during the proliferation cycle with a maximal activity between day 2 and day 4 of culture growth Glycine max ?
-
?

Subunits

Subunits Comment Organism
dimer 1 * 26000 + 1 * 29000, SDS-PAGE Glycine max