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Literature summary for 4.4.1.5 extracted from

  • Deswal, R.; Sopory, S.K.
    Purification and partial characterization of glyoxalase I from a higher plant Brassica juncea (1991), FEBS Lett., 282, 277-280.
    View publication on PubMed

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ potent stimulator, optimal concentration: 16 mM Brassica juncea

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
27000
-
x * 27000, SDS-PAGE Brassica juncea

Organism

Organism UniProt Comment Textmining
Brassica juncea
-
no isoenzymes
-

Purification (Commentary)

Purification (Comment) Organism
-
Brassica juncea

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
188.3
-
-
Brassica juncea

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glutathione + methylglyoxal the substrat is the hemithioacetal of methylglyoxal and glutathione Brassica juncea S-lactoylglutathione
-
?

Subunits

Subunits Comment Organism
? x * 27000, SDS-PAGE Brassica juncea

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8
-
-
Brassica juncea

pH Range

pH Minimum pH Maximum Comment Organism
5.8 8.2 pH 5.8: about 30% of maximal activity, pH 8.2: about 85% of maximal activity Brassica juncea