Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | 10 mM, no inhibition | Pseudomonas putida | |
Zn2+ | 0.1 mM, 75% inhibition at 0.1 mM, complete inhibition at 1.0 mM | Pseudomonas putida |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.2 | - |
4,5-Dioxovalerate | - |
Pseudomonas putida | |
3.5 | - |
methylglyoxal | - |
Pseudomonas putida |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
20000 | - |
1 * 20000, SDS-PAGE | Pseudomonas putida |
21000 | - |
gel filtration | Pseudomonas putida |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Pseudomonas putida |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
359.7 | - |
- |
Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glutathione + 4,5-dioxovalerate | - |
Pseudomonas putida | ? | - |
? | |
glutathione + methylglyoxal | - |
Pseudomonas putida | S-lactoylglutathione | - |
? | |
glutathione + phenylglyoxal | - |
Pseudomonas putida | S-mandeloylglutathione | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 20000, SDS-PAGE | Pseudomonas putida |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Pseudomonas putida |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 9 | 50% of maximal activity at pH 5.0 and at pH 9.0 | Pseudomonas putida |