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Literature summary for 4.4.1.19 extracted from

  • Graham, D.E.; Xu, H.; White, R.H.
    Identification of coenzyme M biosynthetic phosphosulfolactate synthase: a new family of sulfonate-biosynthesizing enzymes (2002), J. Biol. Chem., 277, 13421-13429.
    View publication on PubMed
Search for more literature for 4.4.1.19

Protein Variants

Protein Variants Comment Organism
K137N no measurable enzymatic activity Methanocaldococcus jannaschii

Inhibitors

Inhibitors Comment Organism Structure
EDTA
-
 Methanocaldococcus jannaschii
LiCl weak Methanocaldococcus jannaschii
MgCl2 inhibition above 10 mM Methanocaldococcus jannaschii
additional information further weak inhibitors, not inhibitory: K+, Na+, NH4+ Methanocaldococcus jannaschii
phosphoenolpyruvate substrate inhibition above 5 mM Methanocaldococcus jannaschii
sulfite substrate inhibition above 3 mM Methanocaldococcus jannaschii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
9.8
-
 phosphoenolpyruvate
-
 Methanocaldococcus jannaschii

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Methanocaldococcus jannaschii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
28000
-
 3 * 32000, SDS-PAGE, 3 * 28000, deduced from gene sequence Methanocaldococcus jannaschii
32000
-
 3 * 32000, SDS-PAGE, 3 * 28000, deduced from gene sequence Methanocaldococcus jannaschii
86700
-
 gel filtration Methanocaldococcus jannaschii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Methanocaldococcus jannaschii catalyzes the first step in coenzyme M biosynthesis ?
-
 ?
sulfite + phosphoenolpyruvate Methanocaldococcus jannaschii
-
 (2R)-O-phospho-3-sulfolactate
-
 ?

Organism

Organism UniProt Comment Textmining
Methanocaldococcus jannaschii Q57703
-
-

Reaction

Reaction Comment Organism Reaction ID
(2R)-2-O-phospho-3-sulfolactate = phosphoenolpyruvate + sulfite catalyses the Michael addition of sulfite to phosphoenolpyruvate, requires Mg2+, specifically requires phosphoenolpyruvate and its broad alkaline pH optimum suggests that it uses sulfite rather than bisulfite Methanocaldococcus jannaschii
a

Renatured (Commentary)

Renatured (Comment) Organism
5 mM MgCl2 restores activity of EDTA-treated enzyme Methanocaldococcus jannaschii

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4
-
 recombinant enzyme Methanocaldococcus jannaschii

Storage Stability

Storage Stability Organism
4°C, 20 mM Tris-HCl, 1 mM MgCl2, pH 8.0, 2 months, no loss of activity Methanocaldococcus jannaschii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information catalyzes the first step in coenzyme M biosynthesis Methanocaldococcus jannaschii ?
-
 ?
sulfite + phosphoenolpyruvate
-
 Methanocaldococcus jannaschii (2R)-O-phospho-3-sulfolactate
-
 ?
sulfite + phosphoenolpyruvate
-
 Methanocaldococcus jannaschii (2R)-O-phospho-3-sulfolactate enantiomeric ratio of 84:16 R-isomer:S-isomer ir

Subunits

Subunits Comment Organism
trimer 3 * 32000, SDS-PAGE, 3 * 28000, deduced from gene sequence Methanocaldococcus jannaschii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
 Methanocaldococcus jannaschii

pH Range

pH Minimum pH Maximum Comment Organism
6 11
-
 Methanocaldococcus jannaschii

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.9
-
 phosphoenolpyruvate
-
 Methanocaldococcus jannaschii