Cloned (Comment) | Organism |
---|---|
recombinant expression in Escherichia coli strain BL21(DE3) | Rattus norvegicus |
recombinant expression of GFP-tagged enzyme in spermatids of mature testes of transgenic mice | Mus musculus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | HeLa cells transfected with enzyme-specific siRNA show decreased seleno-labeling efficiency of selenoproteins with selenoprotein P compared to the wild-type | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
nucleus | - |
Mus musculus | 5634 | - |
nucleus | - |
Homo sapiens | 5634 | - |
nucleus | - |
Rattus norvegicus | 5634 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-selenocysteine + reduced acceptor | Mus musculus | - |
selenide + L-alanine + acceptor | - |
? | |
L-selenocysteine + reduced acceptor | Homo sapiens | - |
selenide + L-alanine + acceptor | - |
? | |
L-selenocysteine + reduced acceptor | Rattus norvegicus | - |
selenide + L-alanine + acceptor | - |
? | |
L-selenocysteine + reduced acceptor | Mus musculus C57/BL6 | - |
selenide + L-alanine + acceptor | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Mus musculus | - |
- |
- |
Mus musculus C57/BL6 | - |
- |
- |
Rattus norvegicus | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
HeLa cell | - |
Homo sapiens | - |
kidney | - |
Rattus norvegicus | - |
kidney | high expression level in nuclei of renal proximal tubules, less in distal tubules, no expression in glomerulus cells | Mus musculus | - |
Leydig cell | - |
Mus musculus | - |
Leydig cell | - |
Homo sapiens | - |
Leydig cell | - |
Rattus norvegicus | - |
liver | - |
Mus musculus | - |
liver | - |
Rattus norvegicus | - |
additional information | tissue expression pattern, cell-specific expression in kidney and liver, overview | Mus musculus | - |
seminiferous tubule | dominantly in Leydig cells and spermatids | Mus musculus | - |
seminiferous tubule | dominantly in Leydig cells and spermatids | Homo sapiens | - |
seminiferous tubule | dominantly in Leydig cells and spermatids | Rattus norvegicus | - |
Sertoli cell | low enzyme level | Mus musculus | - |
spermatid | - |
Homo sapiens | - |
spermatid | - |
Rattus norvegicus | - |
spermatid | high enzyme content and activity | Mus musculus | - |
testis | - |
Mus musculus | - |
testis | - |
Homo sapiens | - |
testis | - |
Rattus norvegicus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-selenocysteine + reduced acceptor | - |
Mus musculus | selenide + L-alanine + acceptor | - |
? | |
L-selenocysteine + reduced acceptor | - |
Homo sapiens | selenide + L-alanine + acceptor | - |
? | |
L-selenocysteine + reduced acceptor | - |
Rattus norvegicus | selenide + L-alanine + acceptor | - |
? | |
L-selenocysteine + reduced acceptor | - |
Mus musculus C57/BL6 | selenide + L-alanine + acceptor | - |
? | |
additional information | the enzyme is specific for L-selenocysteine versus L-cysteine on a molecular level, no activity with L-cysteine | Mus musculus | ? | - |
? | |
additional information | the enzyme is specific for L-selenocysteine versus L-cysteine on a molecular level, no activity with L-cysteine | Homo sapiens | ? | - |
? | |
additional information | the enzyme is specific for L-selenocysteine versus L-cysteine on a molecular level, no activity with L-cysteine. Residue Cys375 sorts selenium from cysteine and directs it to the active site | Rattus norvegicus | ? | - |
? | |
additional information | the enzyme is specific for L-selenocysteine versus L-cysteine on a molecular level, no activity with L-cysteine | Mus musculus C57/BL6 | ? | - |
? |
Organism | Comment | Expression |
---|---|---|
Homo sapiens | presence or absence of selenoproteins does not affect enzyme expression in HeLa cells | additional information |
General Information | Comment | Organism |
---|---|---|
additional information | the enzyme is involved in selenocysteine recovering from selenoproteins to be used for synthesis of new selenoproteins | Rattus norvegicus |