Any feedback?
Literature summary for 4.4.1.16 extracted from
- Selenocysteine lyase from mouse liver (2002), Methods Enzymol., 347, 198-203.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) | Mus musculus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| hydroxylamine | almost completely reversible by dialysis against pyridoxal 5'-phosphate | Mus musculus |  |
| NaBH4 | irreversible | Mus musculus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 8.6 | - |
L-cysteine sulfinate | pH 9.0, 37°C | Mus musculus | |
| 9.9 | - |
L-selenocysteine | pH 9.0, 37°C | Mus musculus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | - |
Mus musculus | 5737 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 47000 | - |
SDS-PAGE | Mus musculus |
| 47200 | - |
calculated from amino acid sequence | Mus musculus |
| 105000 | - |
gel filtration | Mus musculus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-selenocysteine + reduced acceptor | Mus musculus | - |
selenide + L-alanine + acceptor | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mus musculus | Q9JLI6 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| overexpressed protein | Mus musculus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| brain | - |
Mus musculus | - |
| heart | - |
Mus musculus | - |
| kidney | - |
Mus musculus | - |
| liver | - |
Mus musculus | - |
| lung | - |
Mus musculus | - |
| spleen | - |
Mus musculus | - |
| stomach | - |
Mus musculus | - |
| testis | - |
Mus musculus | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -80°C, purified enzyme, stable for several weeks | Mus musculus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-cysteine sulfinic acid + reduced acceptor | - |
Mus musculus | SO2 + L-alanine + acceptor | - |
? | |
| L-selenocysteine + reduced acceptor | - |
Mus musculus | selenide + L-alanine + acceptor | - |
? | |
| additional information | traces of activity with L-cysteine | Mus musculus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 47000, SDS-PAGE | Mus musculus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.35 | - |
L-cysteine sulfinate | pH 9.0, 37°C | Mus musculus | |
| 46 | - |
L-selenocysteine | pH 9.0, 37°C | Mus musculus | |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| pyridoxal 5'-phosphate | required | Mus musculus | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
