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Literature summary for 4.3.3.7 extracted from

  • Erzeel, E.; Van Bochaute, P.; Thu, T.T.; Angenon, G.
    Medicago truncatula dihydrodipicolinate synthase (DHDPS) enzymes display novel regulatory properties (2013), Plant Mol. Biol., 81, 401-415.
    View publication on PubMed
Search for more literature for 4.3.3.7

Cloned(Commentary)

Cloned (Comment) Organism
isozymes MtDHDPS, MtDHDPS2, MtDHDPS3, and MtDHDPS4, phylogenetic analysis, quantitative RT-PCR expression analysis, the different isogenes are transcriptionally regulated in an organ-specific manner, subcloning in Escherichia coli strain DH5alpha, expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3), coexpresion of His-tagged isozyme MtDHDPS3 with N-terminally FLAG-tagged enzyme AtDHDPS2 from Arabidopsis thliana in Escherichia coli strain BL21(DE3), expression of isozyme MtDHDPS3 in transgenic Arabidopsis thaliana plants under the control of a constitutive 35S promoter via Agrobacterium tumefaciens transfection resulting in an inhibition of enzyme activity Medicago truncatula

Inhibitors

Inhibitors Comment Organism Structure
L-lysine feedback inhibition Medicago truncatula

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
pyruvate + L-aspartate-4-semialdehyde Medicago truncatula
-
 (2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + H2O
-
 ?
pyruvate + L-aspartate-4-semialdehyde Medicago truncatula Jemalong 2HA
-
 (2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Medicago truncatula
-
 four isozymes, MtDHDPS, MtDHDPS2, MtDHDPS3, and MtDHDPS4
-
Medicago truncatula Jemalong 2HA
-
 four isozymes, MtDHDPS, MtDHDPS2, MtDHDPS3, and MtDHDPS4
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged isozymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Medicago truncatula

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Medicago truncatula
-
additional information organ-specific isozyme expression Medicago truncatula
-
root isozyme MtDHDPS2 shows highest expression in roots Medicago truncatula
-
seed isozymes MtDHDPS1, MtDHDPS3, and MtDHDPS4 shows highest expression in mature seeds Medicago truncatula
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information isozyme MtDHDPS1, followed by isozyme MtDHDPS4, exhibits the highest activity, while activity of isozyme MtDHDPS2 and especially of isozyme MtDHDPS3 is low Medicago truncatula ?
-
 ?
additional information isozyme MtDHDPS1, followed by isozyme MtDHDPS4, exhibits the highest activity, while activity of isozyme MtDHDPS2 and especially of isozyme MtDHDPS3 is low Medicago truncatula Jemalong 2HA ?
-
 ?
pyruvate + L-aspartate-4-semialdehyde
-
 Medicago truncatula (2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + H2O
-
 ?
pyruvate + L-aspartate-4-semialdehyde
-
 Medicago truncatula Jemalong 2HA (2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + H2O
-
 ?

Synonyms

Synonyms Comment Organism
DHDPS
-
 Medicago truncatula
dihydrodipicolinate synthase
-
 Medicago truncatula

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Medicago truncatula

General Information

General Information Comment Organism
metabolism lysine biosynthesis in plants is tightly regulated by feedback inhibition of the end product on dihydrodipicolinate synthase, the first enzyme of the lysine-specific branch Medicago truncatula