Cloned (Comment) | Organism |
---|---|
isozymes MtDHDPS, MtDHDPS2, MtDHDPS3, and MtDHDPS4, phylogenetic analysis, quantitative RT-PCR expression analysis, the different isogenes are transcriptionally regulated in an organ-specific manner, subcloning in Escherichia coli strain DH5alpha, expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3), coexpresion of His-tagged isozyme MtDHDPS3 with N-terminally FLAG-tagged enzyme AtDHDPS2 from Arabidopsis thliana in Escherichia coli strain BL21(DE3), expression of isozyme MtDHDPS3 in transgenic Arabidopsis thaliana plants under the control of a constitutive 35S promoter via Agrobacterium tumefaciens transfection resulting in an inhibition of enzyme activity | Medicago truncatula |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
L-lysine | feedback inhibition | Medicago truncatula |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
pyruvate + L-aspartate-4-semialdehyde | Medicago truncatula | - |
(2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + H2O | - |
? | |
pyruvate + L-aspartate-4-semialdehyde | Medicago truncatula Jemalong 2HA | - |
(2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Medicago truncatula | - |
four isozymes, MtDHDPS, MtDHDPS2, MtDHDPS3, and MtDHDPS4 | - |
Medicago truncatula Jemalong 2HA | - |
four isozymes, MtDHDPS, MtDHDPS2, MtDHDPS3, and MtDHDPS4 | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged isozymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Medicago truncatula |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Medicago truncatula | - |
additional information | organ-specific isozyme expression | Medicago truncatula | - |
root | isozyme MtDHDPS2 shows highest expression in roots | Medicago truncatula | - |
seed | isozymes MtDHDPS1, MtDHDPS3, and MtDHDPS4 shows highest expression in mature seeds | Medicago truncatula | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | isozyme MtDHDPS1, followed by isozyme MtDHDPS4, exhibits the highest activity, while activity of isozyme MtDHDPS2 and especially of isozyme MtDHDPS3 is low | Medicago truncatula | ? | - |
? | |
additional information | isozyme MtDHDPS1, followed by isozyme MtDHDPS4, exhibits the highest activity, while activity of isozyme MtDHDPS2 and especially of isozyme MtDHDPS3 is low | Medicago truncatula Jemalong 2HA | ? | - |
? | |
pyruvate + L-aspartate-4-semialdehyde | - |
Medicago truncatula | (2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + H2O | - |
? | |
pyruvate + L-aspartate-4-semialdehyde | - |
Medicago truncatula Jemalong 2HA | (2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
DHDPS | - |
Medicago truncatula |
dihydrodipicolinate synthase | - |
Medicago truncatula |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Medicago truncatula |
General Information | Comment | Organism |
---|---|---|
metabolism | lysine biosynthesis in plants is tightly regulated by feedback inhibition of the end product on dihydrodipicolinate synthase, the first enzyme of the lysine-specific branch | Medicago truncatula |