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Literature summary for 4.3.3.7 extracted from

  • Siddiqui, T.; Paxman, J.J.; Dogovski, C.; Panjikar, S.; Perugini, M.A.
    Cloning to crystallization of dihydrodipicolinate synthase from the intracellular pathogen Legionella pneumophila (2013), Acta Crystallogr. Sect. F, 69, 1177-1181.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.3.3.7

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and analysis, recombinant expression in Escherichia coli strain BL21(DE3) Legionella pneumophila

Crystallization (Commentary)

Crystallization (Comment) Organism
purified recombinant enzyme, hanging drop vapour diffusion method, mixing of 0.002 ml of 11 mg/ml protein in 20 mM Tris, pH 7.5, with 0.002 ml of reservoir solution containing 0.2 M magnesium chloride, 10% w/v PEG 8000, 0.1 M Tris chloride, pH 10, at 8°C, X-ray diffraction structure determination and analysis at 1.65-1.74 A resolution, molecular replacement Legionella pneumophila

Localization

Localization Comment Organism GeneOntology No. Textmining

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(S)-aspartate-4-semialdehyde + pyruvate Legionella pneumophila
-
 (2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinic acid + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Legionella pneumophila
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme from Escherichia coli strain BL21(DE3) by anion exchange and hydrophobic interaction chromatography, followed by dialysis Legionella pneumophila

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-aspartate-4-semialdehyde + pyruvate
-
 Legionella pneumophila (2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinic acid + H2O
-
 ?

Synonyms

Synonyms Comment Organism
DHDPS
-
 Legionella pneumophila
dihydrodipicolinate synthase
-
 Legionella pneumophila

General Information

General Information Comment Organism
metabolism the enzyme catalyses the first committed step in the lysine biosynthesis pathway, the condensation of pyruvate and (S)-aspartate semialdehyde to form (4S)-4-hydroxy-2,3,4,5-tetrahydro-(2S)-dipicolinic acid Legionella pneumophila