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Literature summary for 4.3.3.7 extracted from

  • Griffin, M.D.; Billakanti, J.M.; Gerrard, J.A.; Dobson, R.C.; Pearce, F.G.
    Crystallization and preliminary X-ray diffraction analysis of dihydrodipicolinate synthase 2 from Arabidopsis thaliana (2011), Acta Crystallogr. Sect. F, 67, 1386-1390.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
orf AT2G45440, recombinant His-tagged enzyme expression in Escherichia coli strain BL21 (DE3) Star Arabidopsis thaliana

Crystallization (Commentary)

Crystallization (Comment) Organism
purified recombinant His-tagged enzyme, sitting drop vapour diffusion method, 0.0015 ml of 14.5 mg/ml protein in 20 mM Tris-HCl, pH 8.0 is mixed with 0.0015 ml of reservoir solution containing 20% w/v PEG 6000, 200 mM sodium chloride, 100 mM Tris-HCl, pH 8.0, including 0.02% w/v sodium azide, 20°C, method screening, 5 days to 8 weeks, X-ray diffraction structure determination and analysis at 2.5 A resolution Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast
-
Arabidopsis thaliana 9507
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
34680
-
x * 34680, mass spectrometry Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(S)-aspartate-4-semialdehyde + pyruvate Arabidopsis thaliana
-
(2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinic acid + H2O
-
?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q9FVC8
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21 (DE3) Star by nickel affinity chromatography, cleavage of the His-tag by TEV protease Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-aspartate-4-semialdehyde + pyruvate
-
Arabidopsis thaliana (2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinic acid + H2O
-
?

Subunits

Subunits Comment Organism
? x * 34680, mass spectrometry Arabidopsis thaliana

Synonyms

Synonyms Comment Organism
AT2G45440
-
Arabidopsis thaliana
DHDPS2
-
Arabidopsis thaliana
dihydrodipicolinate synthase
-
Arabidopsis thaliana

General Information

General Information Comment Organism
metabolism the enzyme catalyzes the branch-point reaction in the biosynthetic pathway leading to meso-diaminopimelate and (S)-lysine in plants and bacteria: condensation of (S)-aspartate-4-semialdehyde and pyruvate to form (4S)-4-hydroxy-2,3,4,5-tetrahydro-(2S)-dipicolinic acid. There are four variants of the meso-diaminopimelate/(S)-lysine pathway, they all share the same enzymatic steps for the synthesis of tetrahydrodipicolinate from aspartate, which includes the reaction catalyzed by the enzyme, overview Arabidopsis thaliana