Cloned (Comment) | Organism |
---|---|
orf AT2G45440, recombinant His-tagged enzyme expression in Escherichia coli strain BL21 (DE3) Star | Arabidopsis thaliana |
Crystallization (Comment) | Organism |
---|---|
purified recombinant His-tagged enzyme, sitting drop vapour diffusion method, 0.0015 ml of 14.5 mg/ml protein in 20 mM Tris-HCl, pH 8.0 is mixed with 0.0015 ml of reservoir solution containing 20% w/v PEG 6000, 200 mM sodium chloride, 100 mM Tris-HCl, pH 8.0, including 0.02% w/v sodium azide, 20°C, method screening, 5 days to 8 weeks, X-ray diffraction structure determination and analysis at 2.5 A resolution | Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | - |
Arabidopsis thaliana | 9507 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
34680 | - |
x * 34680, mass spectrometry | Arabidopsis thaliana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-aspartate-4-semialdehyde + pyruvate | Arabidopsis thaliana | - |
(2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinic acid + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | Q9FVC8 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain BL21 (DE3) Star by nickel affinity chromatography, cleavage of the His-tag by TEV protease | Arabidopsis thaliana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-aspartate-4-semialdehyde + pyruvate | - |
Arabidopsis thaliana | (2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinic acid + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 34680, mass spectrometry | Arabidopsis thaliana |
Synonyms | Comment | Organism |
---|---|---|
AT2G45440 | - |
Arabidopsis thaliana |
DHDPS2 | - |
Arabidopsis thaliana |
dihydrodipicolinate synthase | - |
Arabidopsis thaliana |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme catalyzes the branch-point reaction in the biosynthetic pathway leading to meso-diaminopimelate and (S)-lysine in plants and bacteria: condensation of (S)-aspartate-4-semialdehyde and pyruvate to form (4S)-4-hydroxy-2,3,4,5-tetrahydro-(2S)-dipicolinic acid. There are four variants of the meso-diaminopimelate/(S)-lysine pathway, they all share the same enzymatic steps for the synthesis of tetrahydrodipicolinate from aspartate, which includes the reaction catalyzed by the enzyme, overview | Arabidopsis thaliana |