Literature summary for 4.3.3.7 extracted from

Kang, B.S.; Kim, Y.G.; Ahn, J.W.; Kim, K.J.
Crystal structure of dihydrodipicolinate synthase from Hahella chejuensis at 1.5 A resolution (2010), Int. J. Biol. Macromol., 46, 512-516.

Application

Application	Comment	Organism
industry	considering the industrial application of this protein, such as its use for lysine biosynthesis, stable conformation via tight tetramerization interfaces may make this valuable protein to be more useful	Hahella chejuensis

Cloned(Commentary)

Cloned (Comment)	Organism
ligated into the expression vector pET30a and transformed into the Escherichia coli strain B834 for overexpression	Hahella chejuensis

Crystallization (Commentary)

Crystallization (Comment)	Organism
at 239K using the hanging drop-vapor diffusion method, at 1.5 A resolution. The four subunits of the asymmetric unit assemble to form a tetramer with an approximate 222 symmetry. At the active site, three residues Tyr132, Thr43 and Tyr106 are observed to constitute a catalytic triad. Has a unique extensive dimerdimer interface that is mediated by strong hydrophobic interactions supplemented by two sets of three hydrogen bonds between four polar residues. Belongs to space group P2(1)2(1)2(1) with cell parameters a = 67.03 A, b = 120.52 A, c = 161.1 A	Hahella chejuensis

Crystallization (Comment)

Organism

at 239K using the hanging drop-vapor diffusion method, at 1.5 A resolution. The four subunits of the asymmetric unit assemble to form a tetramer with an approximate 222 symmetry. At the active site, three residues Tyr132, Thr43 and Tyr106 are observed to constitute a catalytic triad. Has a unique extensive dimerdimer interface that is mediated by strong hydrophobic interactions supplemented by two sets of three hydrogen bonds between four polar residues. Belongs to space group P2(1)2(1)2(1) with cell parameters a = 67.03 A, b = 120.52 A, c = 161.1 A

Hahella chejuensis

Organism

Organism	UniProt	Comment	Textmining
Hahella chejuensis	Q2S9K4	-	-

Purification (Commentary)

Purification (Comment)	Organism
by centrifugation and gel filtration	Hahella chejuensis

Subunits

Subunits	Comment	Organism
tetramer	crystallography	Hahella chejuensis

Synonyms

Synonyms	Comment	Organism
DHDPS	-	Hahella chejuensis
dihydrodipicolinate synthase	-	Hahella chejuensis

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Release 2023.1 (January 2023)