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Literature summary for 4.3.2.2 extracted from

  • Sivendran, S.; Patterson, D.; Spiegel, E.; McGown, I.; Cowley, D.; Colman, R.F.
    Two novel mutant human adenylosuccinate lyases (ASLs) associated with autism and characterization of the equivalent mutant Bacillus subtilis ASL (2004), J. Biol. Chem., 279, 53789-53797.
    View publication on PubMed
Search for more literature for 4.3.2.2

Cloned(Commentary)

Cloned (Comment) Organism
-
 Bacillus subtilis

Protein Variants

Protein Variants Comment Organism
D65R mutant with differences in thermal stability, catalytic activity and substrate binding Bacillus subtilis
D65R/D69E mutant with differences in thermal stability, catalytic activity and substrate binding Bacillus subtilis
D69E mutant with differences in thermal stability, catalytic activity and substrate binding Bacillus subtilis
E80D/D87E australian patient with autism Homo sapiens
I62D mutant with differences in thermal stability, catalytic activity and substrate binding Bacillus subtilis
I62D/D65R mutant with differences in thermal stability, catalytic activity and substrate binding Bacillus subtilis
I62D/D65R/D69E mutant with differences in thermal stability, catalytic activity and substrate binding Bacillus subtilis
I62D/D69E mutant with differences in thermal stability, catalytic activity and substrate binding Bacillus subtilis
I62E mutant with differences in thermal stability, catalytic activity and substrate binding Bacillus subtilis
I62E/D65R "humanized" normal enzyme Bacillus subtilis
I62E/D65R/D69E mutant with differences in thermal stability, catalytic activity and substrate binding Bacillus subtilis
I62E/D69E mutant with differences in thermal stability, catalytic activity and substrate binding Bacillus subtilis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0013
-
 adenylsuccinate mutant D69E, 50 mM HEPES, pH 7.0, 25°C Bacillus subtilis
0.002
-
 adenylsuccinate mutant D65R/D69E, 50 mM HEPES, pH 7.0, 25°C Bacillus subtilis
0.004
-
 adenylsuccinate 50 mM HEPES, pH 7.0, 25°C Homo sapiens
0.0043
-
 adenylsuccinate mutant I62E/D69E, 50 mM HEPES, pH 7.0, 25°C Bacillus subtilis
0.0046
-
 adenylsuccinate mutant D65R, 50 mM HEPES, pH 7.0, 25°C Bacillus subtilis
0.0061
-
 adenylsuccinate mutant I62E/D65R/D69E, 50 mM HEPES, pH 7.0, 25°C Bacillus subtilis
0.0107
-
 adenylsuccinate mutant I62D/D65R/D69E, 50 mM HEPES, pH 7.0, 25°C Bacillus subtilis
0.0175
-
 adenylsuccinate mutant I62E, 50 mM HEPES, pH 7.0, 25°C Bacillus subtilis
0.0177
-
 adenylsuccinate mutant I62D/D69E, 50 mM HEPES, pH 7.0, 25°C Bacillus subtilis
0.0181
-
 adenylsuccinate mutant I62E/D65R, 50 mM HEPES, pH 7.0, 25°C Bacillus subtilis
0.0488
-
 adenylsuccinate mutant I62D/D65R, 50 mM HEPES, pH 7.0, 25°C Bacillus subtilis
0.0551
-
 adenylsuccinate mutant I62D, 50 mM HEPES, pH 7.0, 25°C Bacillus subtilis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
155000
-
 mutant D69E, light scattering method, 0.25 mg/ml Bacillus subtilis
159000
-
 mutant I62E/D65R/D69E, light scattering method, 0.25 mg/ml Bacillus subtilis
173000
-
 mutant I62E, light scattering method, 0.25 mg/ml Bacillus subtilis
176000
-
 mutant I62D, light scattering method, 0.25 mg/ml Bacillus subtilis
181000
-
 light scattering method, 0.25 mg/ml Homo sapiens
209000
-
 mutant I62E/D65R, native PAGE Bacillus subtilis
210000
-
 native PAGE Homo sapiens
217000
-
 mutant I62D/D65R, native PAGE Bacillus subtilis
234000
-
 mutant I62D/D65R/D69E, light scattering method, 0.25 mg/ml Bacillus subtilis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5-aminoimidazole-4-(N-succinylcarboxamide) ribonucleotide Bacillus subtilis
-
 5-aminoimidazole-4-carboxamide ribonucleotide + fumarate
-
 ?
5-aminoimidazole-4-(N-succinylcarboxamide) ribonucleotide Homo sapiens
-
 5-aminoimidazole-4-carboxamide ribonucleotide + fumarate
-
 ?
adenylosuccinate Bacillus subtilis
-
 AMP + fumarate
-
 ?
adenylosuccinate Homo sapiens
-
 AMP + fumarate
-
 ?

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
-
-
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Bacillus subtilis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.063
-
 mutant I62D/D65R, adenylsuccinate, 50 mM HEPES, pH 7.0, 25°C Bacillus subtilis
0.069
-
 mutant I62D/D65R, succinylaminoimidazole carboxamide, 50 mM HEPES, pH 7.0, 25°C Bacillus subtilis
0.072
-
 mutant I62D/D65R/D69E, adenylsuccinate, 50 mM HEPES, pH 7.0, 25°C Bacillus subtilis
0.094
-
 mutant I62D/D65R/D69E, succinylaminoimidazole carboxamide, 50 mM HEPES, pH 7.0, 25°C Bacillus subtilis
0.18
-
 mutant I62D/D65R + I62E/D65R/D69E, adenylsuccinate, 50 mM HEPES, pH 7.0, 25°C Bacillus subtilis
0.24
-
 mutant I62D/D65R + I62E/D65R/D69E, succinylaminoimidazole carboxamide, 50 mM HEPES, pH 7.0, 25°C Bacillus subtilis
0.33
-
 mutant I62E/D65R/D69E, adenylsuccinate, 50 mM HEPES, pH 7.0, 25°C Bacillus subtilis
0.46
-
 mutant I62E/D65R/D69E, succinylaminoimidazole carboxamide, 50 mM HEPES, pH 7.0, 25°C Bacillus subtilis
0.53
-
 mutant I62E/D65R/D69E, succinylaminoimidazole carboxamide, 50 mM HEPES, pH 7.0, 25°C Bacillus subtilis
0.54
-
 mutant I62E/D65R, adenylsuccinate, 50 mM HEPES, pH 7.0, 25°C Bacillus subtilis
0.97
-
 mutant I62E/D65R/D69E, adenylsuccinate + succinylaminoimidazole carboxamide, 50 mM HEPES, pH 7.0, 25°C Bacillus subtilis
1.07
-
 adenylsuccinate and succinylaminoimidazole carboxamide, 50 mM HEPES, pH 7.0, 25°C Homo sapiens
1.09
-
 mutant I62D/D65R, adenylsuccinate + succinylaminoimidazole carboxamide, 50 mM HEPES, pH 7.0, 25°C Bacillus subtilis
1.29
-
 mutant I62D/D65R + I62E/D65R/D69E, adenylsuccinate + succinylaminoimidazole carboxamide, 50 mM HEPES, pH 7.0, 25°C Bacillus subtilis
1.3
-
 mutant I62D/D65R/D69E, adenylsuccinate + succinylaminoimidazole carboxamide, 50 mM HEPES, pH 7.0, 25°C Bacillus subtilis
1.4
-
 mutant I62E/D65R/D69E, adenylsuccinate + succinylaminoimidazole carboxamide, 50 mM HEPES, pH 7.0, 25°C Bacillus subtilis
1.58
-
 adenylsuccinate, 50 mM HEPES, pH 7.0, 25°C Homo sapiens
1.7
-
 succinylaminoimidazole carboxamide, 50 mM HEPES, pH 7.0, 25°C Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5-aminoimidazole-4-(N-succinylcarboxamide) ribonucleotide
-
 Bacillus subtilis 5-aminoimidazole-4-carboxamide ribonucleotide + fumarate
-
 ?
5-aminoimidazole-4-(N-succinylcarboxamide) ribonucleotide
-
 Homo sapiens 5-aminoimidazole-4-carboxamide ribonucleotide + fumarate
-
 ?
adenylosuccinate
-
 Bacillus subtilis AMP + fumarate
-
 ?
adenylosuccinate
-
 Homo sapiens AMP + fumarate
-
 ?

Subunits

Subunits Comment Organism
homotetramer
-
 Bacillus subtilis
homotetramer
-
 Homo sapiens

Synonyms

Synonyms Comment Organism
ASL
-
 Bacillus subtilis
ASL
-
 Homo sapiens