Cloned (Comment) | Organism |
---|---|
normal and mutant enzyme S413P, expression in Escherichia coli | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
AMP | - |
Homo sapiens | |
fumarate | noncompetitive | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | pH-dependence of Km-value | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50000 | - |
4 * 50000, SDS-PAGE | Homo sapiens |
196000 | - |
wild-type enzyme, gel filtration | Homo sapiens |
201000 | - |
mutant enzyme S413P, gel filtration | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-(N-succino)-5-aminoimidazole-4-carboxamide ribonucleotide | Homo sapiens | reaction in the biosynthesis of AMP | 5-aminoimidazole-4-carboxamide ribonucleotide + fumarate | - |
? | |
N6-(1,2-dicarboxyethyl)AMP | Homo sapiens | reaction in the biosynthesis of AMP | fumarate + AMP | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
normal and mutant enzyme S413P | - |
Purification (Comment) | Organism |
---|---|
normal and mutant enzyme S413P | Homo sapiens |
Storage Stability | Organism |
---|---|
-80°C or -20°C, recombinant enzyme is very unstable regardless of salt or glycerol concentration | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-(N-succino)-5-aminoimidazole-4-carboxamide ribonucleotide | reaction in the biosynthesis of AMP | Homo sapiens | 5-aminoimidazole-4-carboxamide ribonucleotide + fumarate | - |
? | |
N6-(1,2-dicarboxyethyl)AMP | reaction in the biosynthesis of AMP | Homo sapiens | fumarate + AMP | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 50000, SDS-PAGE | Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | pH-dependence of turnover-numbers | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Homo sapiens |