Application | Comment | Organism |
---|---|---|
medicine | comparison of mouse and human enzyme. Overall results suggest that human and mouse APE1s have mostly similar biochemical and biophysical properties. The conclusions of mouse studies to elucidate APE1 biology and its role in carcinogenesis may be extrapolated to apply to human biology | Homo sapiens |
medicine | comparison of mouse and human enzyme. Overall results suggest that human and mouse APE1s have mostly similar biochemical and biophysical properties. The conclusions of mouse studies to elucidate APE1 biology and its role in carcinogenesis may be extrapolated to apply to human biology | Mus musculus |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Homo sapiens |
expression in Escherichia coli | Mus musculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00082 | - |
5'-TCGAGGATCCTGAGCTCGAGTCGACGXTCGCGAATTCTGCGGATCCAAGC-3' | pH 7.5, 37°C | Homo sapiens | |
0.00091 | - |
5'-TCGAGGATCCTGAGCTCGAGTCGACGXTCGCGAATTCTGCGGATCCAAGC-3' | pH 7.5, 37°C | Mus musculus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
KCl | optimum concentration 50-100 mM | Homo sapiens | |
KCl | optimum concentration 50-100 mM | Mus musculus | |
Mg2+ | required. Increasing the Mg2+ concentration alters the ratio of turns to beta-strands, and this change may be associated with the conformational changes required to achieve an active state | Homo sapiens | |
Mg2+ | required. Increasing the Mg2+ concentration alters the ratio of turns to beta-strands, and this change may be associated with the conformational changes required to achieve an active state | Mus musculus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P27695 | - |
- |
Mus musculus | P28352 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5'-TCGAGGATCCTGAGCTCGAGTCGACGXTCGCGAATTCTGCGGATCCAAGC-3' | a synthetic stable AP-site analog where X represents tetrahydrofuran | Homo sapiens | ? | - |
? | |
5'-TCGAGGATCCTGAGCTCGAGTCGACGXTCGCGAATTCTGCGGATCCAAGC-3' | a synthetic stable AP-site analog where X represents tetrahydrofuran | Mus musculus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
APE1 | - |
Homo sapiens |
APE1 | - |
Mus musculus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.2 | - |
5'-TCGAGGATCCTGAGCTCGAGTCGACGXTCGCGAATTCTGCGGATCCAAGC-3' | pH 7.5, 37°C | Homo sapiens | |
4.2 | - |
5'-TCGAGGATCCTGAGCTCGAGTCGACGXTCGCGAATTCTGCGGATCCAAGC-3' | pH 7.5, 37°C | Mus musculus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | 8 | - |
Homo sapiens |
7.5 | 8 | - |
Mus musculus |