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Literature summary for 4.2.99.18 extracted from

  • Adhikari, S.; Manthena, P.V.; Kota, K.K.; Karmahapatra, S.K.; Roy, G.; Saxena, R.; Uren, A.; Roy, R.
    A comparative study of recombinant mouse and human apurinic/apyrimidinic endonuclease (2012), Mol. Cell. Biochem., 362, 195-201.
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
medicine comparison of mouse and human enzyme. Overall results suggest that human and mouse APE1s have mostly similar biochemical and biophysical properties. The conclusions of mouse studies to elucidate APE1 biology and its role in carcinogenesis may be extrapolated to apply to human biology Homo sapiens
medicine comparison of mouse and human enzyme. Overall results suggest that human and mouse APE1s have mostly similar biochemical and biophysical properties. The conclusions of mouse studies to elucidate APE1 biology and its role in carcinogenesis may be extrapolated to apply to human biology Mus musculus

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Homo sapiens
expression in Escherichia coli Mus musculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00082
-
5'-TCGAGGATCCTGAGCTCGAGTCGACGXTCGCGAATTCTGCGGATCCAAGC-3' pH 7.5, 37°C Homo sapiens
0.00091
-
5'-TCGAGGATCCTGAGCTCGAGTCGACGXTCGCGAATTCTGCGGATCCAAGC-3' pH 7.5, 37°C Mus musculus

Metals/Ions

Metals/Ions Comment Organism Structure
KCl optimum concentration 50-100 mM Homo sapiens
KCl optimum concentration 50-100 mM Mus musculus
Mg2+ required. Increasing the Mg2+ concentration alters the ratio of turns to beta-strands, and this change may be associated with the conformational changes required to achieve an active state Homo sapiens
Mg2+ required. Increasing the Mg2+ concentration alters the ratio of turns to beta-strands, and this change may be associated with the conformational changes required to achieve an active state Mus musculus

Organism

Organism UniProt Comment Textmining
Homo sapiens P27695
-
-
Mus musculus P28352
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5'-TCGAGGATCCTGAGCTCGAGTCGACGXTCGCGAATTCTGCGGATCCAAGC-3' a synthetic stable AP-site analog where X represents tetrahydrofuran Homo sapiens ?
-
?
5'-TCGAGGATCCTGAGCTCGAGTCGACGXTCGCGAATTCTGCGGATCCAAGC-3' a synthetic stable AP-site analog where X represents tetrahydrofuran Mus musculus ?
-
?

Synonyms

Synonyms Comment Organism
APE1
-
Homo sapiens
APE1
-
Mus musculus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2
-
5'-TCGAGGATCCTGAGCTCGAGTCGACGXTCGCGAATTCTGCGGATCCAAGC-3' pH 7.5, 37°C Homo sapiens
4.2
-
5'-TCGAGGATCCTGAGCTCGAGTCGACGXTCGCGAATTCTGCGGATCCAAGC-3' pH 7.5, 37°C Mus musculus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5 8
-
Homo sapiens
7.5 8
-
Mus musculus