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Literature summary for 4.2.99.18 extracted from

  • Sartori, A.A.; Lingaraju, G.M.; Hunziker, P.; Winkler, F.K.; Jiricny, J.
    Pa-AGOG, the founding member of a new family of archaeal 8-oxoguanine DNA-glycosylases (2004), Nucleic Acids Res., 32, 6531-6539.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.2.99.18

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Pyrobaculum aerophilum

Protein Variants

Protein Variants Comment Organism
D166N mutant enzyme shows wild-type activity Pyrobaculum aerophilum
D172N inactive mutant enzyme Pyrobaculum aerophilum
K140Q inactive mutant enzyme Pyrobaculum aerophilum
K147Q activity of mutant enzyme is severely attenuated Pyrobaculum aerophilum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
29467
-
 x * 29467, calculated from sequence Pyrobaculum aerophilum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
DNA with an abasic site Pyrobaculum aerophilum the enzyme is part of the base excision repair (BER) pathway. It protects from oxidative damage by removing the major product of DNA oxidation, 8-oxoguanine, from single- and double-stranded DNA substrates ?
-
 ?
DNA with an abasic site Pyrobaculum aerophilum DSM 7523 the enzyme is part of the base excision repair (BER) pathway. It protects from oxidative damage by removing the major product of DNA oxidation, 8-oxoguanine, from single- and double-stranded DNA substrates ?
-
 ?

Organism

Organism UniProt Comment Textmining
Pyrobaculum aerophilum Q8ZVK6
-
-
Pyrobaculum aerophilum DSM 7523 Q8ZVK6
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pyrobaculum aerophilum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DNA with an abasic site the enzyme is part of the base excision repair (BER) pathway. It protects from oxidative damage by removing the major product of DNA oxidation, 8-oxoguanine, from single- and double-stranded DNA substrates Pyrobaculum aerophilum ?
-
 ?
DNA with an abasic site the substrate specificity for 8-oxoguanine/guanine sites is higher than that for 8-oxoguanine/cytosine sites. apurinic-lyase activity of this enzyme cleaved only 20% of these apurinic-sites at the same time point Pyrobaculum aerophilum ?
-
 ?
DNA with an abasic site the enzyme is part of the base excision repair (BER) pathway. It protects from oxidative damage by removing the major product of DNA oxidation, 8-oxoguanine, from single- and double-stranded DNA substrates Pyrobaculum aerophilum DSM 7523 ?
-
 ?
DNA with an abasic site the substrate specificity for 8-oxoguanine/guanine sites is higher than that for 8-oxoguanine/cytosine sites. apurinic-lyase activity of this enzyme cleaved only 20% of these apurinic-sites at the same time point Pyrobaculum aerophilum DSM 7523 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 29467, calculated from sequence Pyrobaculum aerophilum

Synonyms

Synonyms Comment Organism
Pa-AGOG bifunctional enzyme that catalyzes the excision of 8-oxoguanine by cleaving the N-glycosylic bond between the base and the deoxyribose moiety (glycosylase activity) and subsequently cleave the DNA backbone (lyase activity, EC 4.2.99.18) Pyrobaculum aerophilum
PAE2237 locus name Pyrobaculum aerophilum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
80
-
 15 min, no notable effect on catalytic activity Pyrobaculum aerophilum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.2
-
 calculated from sequence Pyrobaculum aerophilum

General Information

General Information Comment Organism
physiological function the enzyme is part of the base excision repair (BER) pathway. It protects from oxidative damage by removing the major product of DNA oxidation, 8-oxoguanine, from single- and double-stranded DNA substrates. Bifunctional enzyme that catalyzes the excision of 8-oxoguanine by cleaving the N-glycosylic bond between the base and the deoxyribose moiety (glycosylase activity) and subsequently cleave the DNA backbone (lyase activity, EC 4.2.99.18) Pyrobaculum aerophilum