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Literature summary for 4.2.99.18 extracted from

  • Gogos, A.; Clarke, N.D.
    Characterization of an 8-oxoguanine DNA glycosylase from Methanococcus jannaschii (1999), J. Biol. Chem., 274, 30447-30450.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Methanocaldococcus jannaschii

Protein Variants

Protein Variants Comment Organism
K129S inactive mutant enzyme Methanocaldococcus jannaschii

Organism

Organism UniProt Comment Textmining
Methanocaldococcus jannaschii Q58134
-
-
Methanocaldococcus jannaschii DSM 2661 Q58134
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Methanocaldococcus jannaschii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DNA with an abasic site the enzyme has little specificity for the base opposite 8-oxoguanine. The enzyme has both DNA glycosylase and DNA lyase (beta-elimination) activity, and the combined glycosylase/lyase activity occurs at a rate comparable with the glycosylase activity alone Methanocaldococcus jannaschii ?
-
?
DNA with an abasic site the enzyme has little specificity for the base opposite 8-oxoguanine. The enzyme has both DNA glycosylase and DNA lyase (beta-elimination) activity, and the combined glycosylase/lyase activity occurs at a rate comparable with the glycosylase activity alone Methanocaldococcus jannaschii DSM 2661 ?
-
?

Synonyms

Synonyms Comment Organism
mjOgg
-
Methanocaldococcus jannaschii
oxoG DNA glycosylase
-
Methanocaldococcus jannaschii

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
37 50 the enzyme is 6fold more active at 50°C than at 37°C, glycosylase/lyase activity is very similarto that of the glycosylase activity alone Methanocaldococcus jannaschii

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
3 h, activity is decreased by one-third Methanocaldococcus jannaschii
93
-
the enzyme is thermally stable, denaturing at 93 °C Methanocaldococcus jannaschii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
assay at Methanocaldococcus jannaschii

pH Range

pH Minimum pH Maximum Comment Organism
7.3 9.5 pH 7.3: about 50% of maximal activity, pH 9.5: about 45% of maximal ativity, glycosylase/lyase activity is very similar to that of the glycosylase activity alone Methanocaldococcus jannaschii