Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 4.2.99.18 extracted from

  • Kim, W.C.; Berquist, B.R.; Chohan, M.; Uy, C.; Wilson, D.M.; Lee, C.H.
    Characterization of the endoribonuclease active site of human apurinic/apyrimidinic endonuclease 1 (2011), J. Mol. Biol., 411, 960-971.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.2.99.18

Protein Variants

Protein Variants Comment Organism
D210N loss of abasic dsDNA incision, complete loss of endoribonuclease activity against c-myc CRD Homo sapiens
D283N loss of abasic dsDNA incision, no change in endoribonuclease activity against c-myc CRD Homo sapiens
D283N retaines endoribonuclease and abasic single-stranded RNA cleavage activities, with concurrent loss of apurinic/apyrimidinic site cleavage activities on double-stranded DNA and single-stranded DNA Homo sapiens
D308A 1.4fold reduction in abasic dsDNA incision, complete loss of endoribonuclease activity against c-myc CRD Homo sapiens
D70A 6.7fold reduction in abasic dsDNA incision, complete loss of endoribonuclease activity against c-myc CRD Homo sapiens
E96A no reduction in abasic dsDNA incision, complete loss of endoribonuclease activity against c-myc CRD Homo sapiens
F266A 30fold reduction in abasic dsDNA incision, complete loss of endoribonuclease activity against c-myc CRD Homo sapiens
H309S loss of abasic dsDNA incision Homo sapiens
N68A loss of abasic dsDNA incision, complete loss of endoribonuclease activity against c-myc CRD Homo sapiens
Y171F loss of abasic dsDNA incision, complete loss of endoribonuclease activity against c-myc CRD Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ both the endoribonuclease and the ssRNA apurinic/apyrimidinic site cleavage activities of wild-type APE1 are present in the absence of Mg2+, while ssDNA apurinic/apyrimidinic site cleavage requires Mg2+, optimally at 0.5-2.0 mM Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information a 2'-OH on the sugar moiety is absolutely required for RNA cleavage by wild-type APE1, consistent with APE1 leaving a 3'-PO4 2? group following cleavage of RNA. the catalytic mechanisms for cleaving RNA, abasic single-stranded RNA, and abasic DNA are not identical Homo sapiens ?
-
 ?