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Literature summary for 4.2.99.18 extracted from

  • Li, M.; Zhong, Z.; Zhu, J.; Xiang, D.; Dai, N.; Cao, X.; Qing, Y.; Yang, Z.; Xie, J.; Li, Z.; Baugh, L.; Wang, G.; Wang, D.
    Identification and characterization of mitochondrial targeting sequence of human apurinic/apyrimidinic endonuclease 1 (2010), J. Biol. Chem., 285, 14871-14881.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
K299A/R301A the mutation diminishes mitochondrial translocation of APE1 Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion the enzyme preferentially resides in the nucleus with conditional distribution in the mitochondria Homo sapiens 5739
-
nucleus the enzyme preferentially resides in the nucleus Homo sapiens 5634
-

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
nickel-Sepharose resin column chromatography, gel filtration Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
HeLa cell
-
Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DNA
-
Homo sapiens fragments of DNA
-
?

Synonyms

Synonyms Comment Organism
APE1
-
Homo sapiens
apurinic/apyrimidinic endonuclease 1
-
Homo sapiens

General Information

General Information Comment Organism
physiological function APE1 functions as a critical rate-limiting enzyme in DNA base excision repair and accounts for nearly all of the AP site incision activities in cell extracts, APE1 also exerts unique redox activity to regulate the DNA-binding affinity of certain transcriptional factors by controlling the redox status of their DNA-binding domain Homo sapiens