Application | Comment | Organism |
---|---|---|
biotechnology | is frequently used in gene technology due to its strong exonucleolytic activity | Archaeoglobus fulgidus |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Archaeoglobus fulgidus |
Crystallization (Comment) | Organism |
---|---|
V217G variant is crystallized with decamer dsDNA molecule, and the three-dimensional structure is determined to 1.7 A resolution | Archaeoglobus fulgidus |
Protein Variants | Comment | Organism |
---|---|---|
F200S | site-directed mutagenesis, by expanding the size of the binding pocket the unspecific endonucleolytic activity is increased | Archaeoglobus fulgidus |
F200S/W215S | site-directed mutagenesis, by expanding the size of the binding pocket the unspecific endonucleolytic activity is increased | Archaeoglobus fulgidus |
V217G | site-directed mutagenesis, by expanding the size of the binding pocket the unspecific endonucleolytic activity is increased | Archaeoglobus fulgidus |
W215S | site-directed mutagenesis, by expanding the size of the binding pocket the unspecific endonucleolytic activity is increased | Archaeoglobus fulgidus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Archaeoglobus fulgidus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Archaeoglobus fulgidus | O29675 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Archaeoglobus fulgidus |
Synonyms | Comment | Organism |
---|---|---|
AP endonuclease | exonuclease III (ExoIII) | Archaeoglobus fulgidus |
apurinic/apyrimidinic endonuclease | - |
Archaeoglobus fulgidus |