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Literature summary for 4.2.99.18 extracted from

  • Mundle, S.T.; Delaney, J.C.; Essigmann, J.M.; Strauss, P.R.
    Enzymatic mechanism of human apurinic/apyrimidinic endonuclease against a THF AP site model substrate (2009), Biochemistry, 48, 19-26.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.2.99.18

Application

Application Comment Organism
drug development inhibition of AP endonuclease may be effective in decreasing the dose requirements of chemotherapeutics used in the treatment of cancer as well as other diseases. Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ 5 mM included in assay medium, required divalent cation Homo sapiens
Mn2+ Glu in amino acid position 96 binds to the divalent cation Homo sapiens
Na+ 65 mM included in assay medium Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
DNA containing apurinic/apyrimidinic site Homo sapiens
-
 DNA fragments
-
 ?
additional information Homo sapiens a DNA base excision repair enzyme with a wide variety of functions, including AP endonuclease (cleaving an AP site 5' to a deoxyribose phosphate moiety), 3' exonuclease, 3' phosphodiesterase, 3' phosphatase, RNaseH, and 5' endonuclease activities ?
-
 ?
additional information Homo sapiens AP endo acts by a one-step associative phosphoryl transfer mechanism on a THF-containing substrate ?
-
 ?
additional information Homo sapiens AP endo acts on many types of DNA substrate molecules but demonstrates the most robust activity when acting as a class II AP endonuclease ?
-
 ?
additional information Homo sapiens major factor in the maintenance of the integrity of the human genome ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 750 nM substrate and 100 nM enzyme and a reaction time of 3 h is sufficient to convert most, if not all, of the Rp stereoisomer substrate DNA into product Homo sapiens
additional information
-
 the enzyme is responsible for eliminating as many as 105 potentially mutagenic and genotoxic lesions from the genome of each cell every day Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DNA containing apurinic/apyrimidinic site
-
 Homo sapiens DNA fragments
-
 ?
DNA containing apurinic/apyrimidinic sites
-
 Homo sapiens ?
-
 ?
additional information a DNA base excision repair enzyme with a wide variety of functions, including AP endonuclease (cleaving an AP site 5' to a deoxyribose phosphate moiety), 3' exonuclease, 3' phosphodiesterase, 3' phosphatase, RNaseH, and 5' endonuclease activities Homo sapiens ?
-
 ?
additional information AP endo acts by a one-step associative phosphoryl transfer mechanism on a THF-containing substrate Homo sapiens ?
-
 ?
additional information AP endo acts on many types of DNA substrate molecules but demonstrates the most robust activity when acting as a class II AP endonuclease Homo sapiens ?
-
 ?
additional information major factor in the maintenance of the integrity of the human genome Homo sapiens ?
-
 ?
additional information AP endo cleaves the Rp but not the Sp stereoisomer of DNA phosphorothioate oligomers, albeit slowly with respect to a phosphodiester substrate Homo sapiens ?
-
 ?
additional information The ratio of AP endo reaction product to STM7 49mer hairpin oligomers is varied. Product formation is maximal with an 8:1 ratio of STM7 acceptor to 5' thiophosphate donor DNA and by addition of extra ATP (0.5 mM) and ligase (60 units) midreaction Homo sapiens ?
-
 ?

Synonyms

Synonyms Comment Organism
AP endo
-
 Homo sapiens
apurinic/apyrimidinic endonuclease
-
 Homo sapiens
HAP1
-
 Homo sapiens
Ref1
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
22
-
 assay at Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
10
-
 DNA containing apurinic/apyrimidinic sites 5' cleavage of a reduced AP site Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Homo sapiens