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Literature summary for 4.2.99.18 extracted from

  • Ho, R.; Rachek, L.I.; Xu, Y.; Kelley, M.R.; LeDoux, S.P.; Wilson, G.L.
    Yeast apurinic/apyrimidinic endonuclease Apn1 protects mammalian neuronal cell line from oxidative stress (2007), J. Neurochem., 102, 13-24.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.2.99.18

Application

Application Comment Organism
medicine Apn1 could be used as a possible treatment strategy for attenuating or slowing down the pathogenesis of neurodegenerative diseases Saccharomyces cerevisiae

Cloned(Commentary)

Cloned (Comment) Organism
the yeast apurinic/apyrimidinic endonuclease gene is cloned into the pcDNA3.neo plasmid for transfection of rat RCSN-3 cells Saccharomyces cerevisiae

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
 Saccharomyces cerevisiae 5739
-
nucleus
-
 Saccharomyces cerevisiae 5634
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40500
-
 Western Blots showing Apn1 expression in purified nuclear, mitochondrial extracts and whole cell lysates Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae P22936
-
-

Purification (Commentary)

Purification (Comment) Organism
nuclear and mitochondrial extracts are isolated Saccharomyces cerevisiae

Synonyms

Synonyms Comment Organism
APN1
-
 Saccharomyces cerevisiae
apurinic/apyrimidinic endonuclease
-
 Saccharomyces cerevisiae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 activity assay, oligonucleotide cleavage assay, endonuclease and 3'-phosphodiesterase activity is measured Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 activity assay, oligonucleotide cleavage assay, endonuclease and 3'-phosphodiesterase activity is measured Saccharomyces cerevisiae