Cloned (Comment) | Organism |
---|---|
into the pET28a vector for expression in Escherichia coli BL21DE3 cells, into the pKK223-3 vector for transformation into the BW528 strain for subsequent complementation studies | Leishmania major |
Crystallization (Comment) | Organism |
---|---|
crystals are produced in hanging drops, the structure is solved at a resolution of 2.5 A | Leishmania major |
Protein Variants | Comment | Organism |
---|---|---|
D335N | mutant retains its capacity to bind to AP sites in DNA, but loses its endonuclease activity | Leishmania major |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | incision activity is completely abolished when 10 mM EDTA is added to the reaction | Leishmania major |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
56000 | - |
- |
Leishmania major |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Leishmania major | O15922 | - |
- |
Purification (Comment) | Organism |
---|---|
using a Ni2+-charged HiTrap Chelating HP column and a gel filtration column | Leishmania major |
Synonyms | Comment | Organism |
---|---|---|
AP endonuclease | - |
Leishmania major |
apurinic/apyrimidinic endonuclease | - |
Leishmania major |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
activity assay | Leishmania major |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
activity assay | Leishmania major |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 8 | - |
Leishmania major |