Cloned (Comment) | Organism |
---|---|
into the pProEX HTb vector for transformation of Escherichia coli XL-1 Blue and ER2566 cells | Neisseria meningitidis |
Crystallization (Comment) | Organism |
---|---|
the crystal structure of NMB2082/NApe is solved at a 1.5 A resolution | Neisseria meningitidis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | the standard reaction buffer used for the nuclease assay is supplemented with 15 mM MgCl2 | Neisseria meningitidis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
33000 | - |
determined by SDS-PAGE and Western blotting | Neisseria meningitidis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Neisseria meningitidis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
using a metal-affinity HisTrap chelating HP column, preequilibrated with CoCl2, and size-exclusion chromatography, using a HiLoad 16/60 Superdex 200 prep grade column | Neisseria meningitidis |
Synonyms | Comment | Organism |
---|---|---|
AP endonuclease | - |
Neisseria meningitidis |
apurinic/apyrimidinic endonuclease | - |
Neisseria meningitidis |
NapE | Neisserial AP endonuclease | Neisseria meningitidis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
nuclease assay | Neisseria meningitidis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
nuclease assay | Neisseria meningitidis |