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Literature summary for 4.2.99.18 extracted from

  • Meadows, K.L.; Song, B.; Doetsch, P.W.
    Characterization of AP lyase activities of Saccharomyces cerevisiae Ntg1p and Ntg2p: implications for biological function (2003), Nucleic Acids Res., 31, 5560-5567.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
Ntg1p and Ntg2p Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0000064
-
37mer with AP/G pH 6.8, 37°C, Ntg2p Saccharomyces cerevisiae
0.0000067
-
37mer with AP/T pH 6.8, 37°C, Ntg1p Saccharomyces cerevisiae
0.0000094
-
37mer with AP/T pH 6.8, 37°C, Ntg2p Saccharomyces cerevisiae
0.000011
-
37mer with AP/A pH 6.8, 37°C, Ntg1p Saccharomyces cerevisiae
0.0000218
-
37mer with AP/A pH 6.8, 37°C, Ntg2p Saccharomyces cerevisiae
0.000025
-
37mer with AP/G pH 6.8, 37°C, Ntg1p Saccharomyces cerevisiae
0.000037
-
37mer with AP/C pH 6.8, 37°C, Ntg1p Saccharomyces cerevisiae
0.000046
-
37mer with AP/C pH 6.8, 37°C, Ntg2p Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
DNA Saccharomyces cerevisiae
-
fragments of DNA
-
?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ntg1p and Ntg2p Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
37mer with AP/A
-
Saccharomyces cerevisiae ?
-
?
37mer with AP/C
-
Saccharomyces cerevisiae ?
-
?
37mer with AP/G
-
Saccharomyces cerevisiae ?
-
?
37mer with AP/T
-
Saccharomyces cerevisiae ?
-
?
DNA
-
Saccharomyces cerevisiae fragments of DNA
-
?
DNA cleaves all four types of abasic site-containing substrates: AP/G, AP/A, AP/C, AP/T, with a moderate preference for AP/T sites Saccharomyces cerevisiae fragments of DNA
-
?
additional information enzyme also has DNA N-glycosylase activity Saccharomyces cerevisiae ?
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0007
-
37mer with AP/A pH 6.8, 37°C, Ntg1p Saccharomyces cerevisiae
0.00108
-
37mer with AP/G pH 6.8, 37°C, Ntg1p Saccharomyces cerevisiae
0.00323
-
37mer with AP/G pH 6.8, 37°C, Ntg2p Saccharomyces cerevisiae
0.00512
-
37mer with AP/A pH 6.8, 37°C, Ntg2p Saccharomyces cerevisiae
0.007
-
37mer with AP/T pH 6.8, 37°C, Ntg1p Saccharomyces cerevisiae
0.015
-
37mer with AP/C pH 6.8, 37°C, Ntg2p Saccharomyces cerevisiae
0.0225
-
37mer with AP/C pH 6.8, 37°C, Ntg1p Saccharomyces cerevisiae
0.0233
-
37mer with AP/T pH 6.8, 37°C, Ntg2p Saccharomyces cerevisiae