Cloned (Comment) | Organism |
---|---|
fusion protein with glutathione S-transferase | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
K212R | lower catalytic specificity than wild-type enzyme | Homo sapiens |
additional information | protein with two additional amino acid residues and a truncated protein with deletion of 22 residues at the amino-terminus are equally active | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 50% inhibition at 5-10 mM | Homo sapiens | |
Mg2+ | 50% inhibition at 5-10 mM | Homo sapiens | |
Mn2+ | 50% inhibition at 5-10 mM | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000069 | - |
DNA containing dihydrouracil | wild-type, pH 8, 37°C | Homo sapiens | |
0.00017 | - |
DNA containing dihydrouracil | K212R mutant, pH 8, 37°C | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Homo sapiens | 5737 | - |
nucleus | - |
Homo sapiens | 5634 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | iron-sulfur protein | Homo sapiens | |
Iron | [4Fe-4S]-cluster | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
36000 | - |
SDS-PAGE | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA | Homo sapiens | - |
fragments of DNA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P78549 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA | - |
Homo sapiens | fragments of DNA | - |
? | |
DNA | cleaves thymine glycol-containing form I plasmid DNA and a dihydrouracil-containing oligonucleotide duplex | Homo sapiens | fragments of DNA | - |
? | |
DNA containing dihydrouracil | - |
Homo sapiens | ? | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0000567 | - |
DNA containing dihydrouracil | K212R mutant, pH 8, 37°C | Homo sapiens | |
0.002 | - |
DNA containing dihydrouracil | wild-type, pH 8, 37°C | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Homo sapiens |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Homo sapiens | - |
- |
10 |