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Literature summary for 4.2.99.18 extracted from
- Isolation and characterization of an apurinic endodeoxyribonuclease from the anaerobic thermophile Desulfotomaculum nigrificans (1984), J. Gen. Microbiol., 130, 1525-1534.
No PubMed abstract available
General Stability
| General Stability | Organism |
|---|---|
| bovine serum albumin protects against heat inactivation | Desulfotomaculum nigrificans |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 4-chloromercuribenzoate | complete inhibition at 1 mM | Desulfotomaculum nigrificans |  |
| EDTA | complete inhibition at 20 mM | Desulfotomaculum nigrificans | |
| Mg2+ | MgCl2 above 10 mM | Desulfotomaculum nigrificans | |
| Mn2+ | above 20 mM | Desulfotomaculum nigrificans | |
| NaCl | above 10 mM; complete inhibition at 500 mM | Desulfotomaculum nigrificans | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | optimal concentration: 10 mM | Desulfotomaculum nigrificans | |
| Mg2+ | Mg2+ or Mn2+ required | Desulfotomaculum nigrificans | |
| Mn2+ | Mn2+ or Mg2+ required | Desulfotomaculum nigrificans | |
| Mn2+ | 10-20 mM | Desulfotomaculum nigrificans | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 18500 | - |
glycerol density gradient sedimentation | Desulfotomaculum nigrificans |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| DNA | Desulfotomaculum nigrificans | - |
fragments of DNA | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Desulfotomaculum nigrificans | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Desulfotomaculum nigrificans |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| vegetative | - |
Desulfotomaculum nigrificans | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Desulfotomaculum nigrificans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| DNA | - |
Desulfotomaculum nigrificans | fragments of DNA | - |
? | |
| DNA | specific for apurinic sites | Desulfotomaculum nigrificans | fragments of DNA | - |
? | |
| additional information | no substrate: alkylated sites | Desulfotomaculum nigrificans | ? | - |
? | |
| additional information | no substrate: native DNA | Desulfotomaculum nigrificans | ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
- |
Desulfotomaculum nigrificans |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | - |
35°C: about 35% of activity maximum, 65°C: about 25% of activity maximum | Desulfotomaculum nigrificans |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
half-life: 55 min, 60 min in presence of bovine serum albumin | Desulfotomaculum nigrificans |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | 8 | - |
Desulfotomaculum nigrificans |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 9 | - |
Desulfotomaculum nigrificans |
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