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Literature summary for 4.2.99.18 extracted from

  • Tomkinson, A.E.; Bonk, R.T.; Linn, S.
    Mitochondrial endonuclease activities specific for apurinic/apyrimidinic sites in DNA from mouse cells (1988), J. Biol. Chem., 263, 12532-12537.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
Triton X-100 no stimulation Mus musculus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
Mus musculus 5737
-
mitochondrion
-
Mus musculus 5739
-

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ stimulation Mus musculus
KCl enzyme form A: optimal activity in 20 mM NaCl or KCl, enzyme form B: more active without salt Mus musculus
Mg2+ stimulates Mus musculus
Mg2+ optimal concentration: 1-5 mM Mus musculus
Mn2+ stimulates Mus musculus
NaCl enzyme form A: optimal activity in 20 mM NaCl or KCl, enzyme form B: more active without salt Mus musculus
Zn2+ stimulation half as effective as with Mg2+ Mus musculus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
61000
-
sedimentation data Mus musculus
65000
-
1 * 65000, SDS-PAGE Mus musculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
DNA Mus musculus
-
fragments of DNA
-
?
additional information Mus musculus role in eliminating damaged mitochondrial genomes from the gene pool ?
-
?

Organism

Organism UniProt Comment Textmining
Mus musculus
-
enzyme forms A and B
-

Purification (Commentary)

Purification (Comment) Organism
-
Mus musculus

Source Tissue

Source Tissue Comment Organism Textmining
MPC-11 cell
-
Mus musculus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
19
-
-
Mus musculus

Storage Stability

Storage Stability Organism
-80°C, pH 8.0, 1 mM EDTA, 0.05% Triton X-100, 0.5 mM dithiothreitol, 10 mM KCl, 3 months Mus musculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DNA
-
Mus musculus fragments of DNA
-
?
DNA supercoiled DNA Mus musculus fragments of DNA
-
?
additional information role in eliminating damaged mitochondrial genomes from the gene pool Mus musculus ?
-
?

Subunits

Subunits Comment Organism
monomer 1 * 65000, SDS-PAGE Mus musculus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5 7.5 enzyme form B Mus musculus
6.5 9 enzyme form A Mus musculus