Protein Variants | Comment | Organism |
---|---|---|
F107A | site-directed mutagenesis, the mutant follows a different reaction mechanism compared to wild-type and produces the different product cembrane A in addition to cyclooctat-9-en-7-ol | Streptomyces melanosporofaciens |
F107Y | site-directed mutagenesis, the mutant follows a different reaction mechanism compared to wild-type and produces the different product cyclooctat-1,7-diene | Streptomyces melanosporofaciens |
F149L | site-directed mutagenesis, the mutant follows a different reaction mechanism compared to wild-type and produces the different product cyclooctat-7-en-3-ol, no formation of cyclooctat-9-en-7-ol | Streptomyces melanosporofaciens |
W288G | site-directed mutagenesis, the mutant follows a different reaction mechanism compared to wild-type and produces the different product 3,7,18-dolabellatriene | Streptomyces melanosporofaciens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
geranylgeranyl diphosphate + H2O | Streptomyces melanosporofaciens | - |
cyclooctat-9-en-7-ol + diphosphate | - |
? | |
geranylgeranyl diphosphate + H2O | Streptomyces melanosporofaciens MI614-43F2 | - |
cyclooctat-9-en-7-ol + diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces melanosporofaciens | C9K1X5 | - |
- |
Streptomyces melanosporofaciens MI614-43F2 | C9K1X5 | - |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
geranylgeranyl diphosphate + H2O = cyclooctat-9-en-7-ol + diphosphate | cyclization reaction mechanism analysis using three regiospecifically deuterated samples of geranylgeranyl diphosphate, the reaction proceeds via several cationic intermediates, detailed overview. Enzyme CotB2 catalyzes an unusually complex regiospecific and stereospecific cyclization that involves a unique carbon-carbon bond rearrangement and multiple hydride shifts, which all take place at a single active site | Streptomyces melanosporofaciens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
geranylgeranyl diphosphate + H2O | - |
Streptomyces melanosporofaciens | cyclooctat-9-en-7-ol + diphosphate | - |
? | |
geranylgeranyl diphosphate + H2O | - |
Streptomyces melanosporofaciens MI614-43F2 | cyclooctat-9-en-7-ol + diphosphate | - |
? | |
additional information | CotB2 has the unique ability to synthesize the characteristic 5-8-5 fused-ring system of cyclooctat-9-en-7-ol having six chiral centers by cyclization of the universal diterpene precursor, the achiral C20 allylic diphosphate GGDP | Streptomyces melanosporofaciens | ? | - |
? | |
additional information | CotB2 has the unique ability to synthesize the characteristic 5-8-5 fused-ring system of cyclooctat-9-en-7-ol having six chiral centers by cyclization of the universal diterpene precursor, the achiral C20 allylic diphosphate GGDP | Streptomyces melanosporofaciens MI614-43F2 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CotB2 | - |
Streptomyces melanosporofaciens |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme catalyzes the formation of cyclooctat-9-en-7-ol, which is a precursor of cyclooctatin, a diterpene with a unique tricyclic diterpene skeleton characterized by a 5-8-5 fused-ring system, which is a potent inhibitor of lysophospholipase, an enzyme that catalyzes the hydrolysis of the fatty acid ester bonds of lysophospholipids | Streptomyces melanosporofaciens |