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Literature summary for 4.2.3.141 extracted from

  • Caniard, A.; Zerbe, P.; Legrand, S.; Cohade, A.; Valot, N.; Magnard, J.L.; Bohlmann, J.; Legendre, L.
    Discovery and functional characterization of two diterpene synthases for sclareol biosynthesis in Salvia sclarea (L.) and their relevance for perfume manufacture (2012), BMC Plant Biol., 12, 119.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.2.3.141

Application

Application Comment Organism
synthesis synthesis of sclareol by co-expression of producing labda-13-en-8-ol diphosphate as major product and manool synthase in Saccharomyces cerevisiae Salvia sclarea

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli and Saccharomyces cerevisiae Salvia sclarea

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast
-
 Salvia sclarea 9507
-
chloroplast enzyme possesses an N-terminal signal sequence that actively targets sclareol synthase to the chloroplast Salvia sclarea 9507
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
61000
-
 x * 61000, SDS-PAGE Salvia sclarea

Organism

Organism UniProt Comment Textmining
Salvia sclarea
-
-
-
Salvia sclarea K4HYB0
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(9S,10S)-copalyl diphosphate + H2O
-
 Salvia sclarea manool + diphosphate
-
 ?
labda-13-en-8-ol diphosphate + H2O
-
 Salvia sclarea sclareol + diphosphate
-
 ?
additional information in coupled enzyme assays with labda-13-en-8-ol diphosphate synthase, sclareol is the major product with minor amounts of manool, manoyl oxides and epi-manoyl oxide as secondary products Salvia sclarea ?
-
 ?
additional information no substrate: geranylgeranyl diphosphate Salvia sclarea ?
-
 ?

Subunits

Subunits Comment Organism
? x * 61000, SDS-PAGE Salvia sclarea

Synonyms

Synonyms Comment Organism
sclareol synthase
-
 Salvia sclarea

General Information

General Information Comment Organism
physiological function enzymes labda-13-en-8-ol diphosphate synthase and sclareol synthase are two monofunctional diterpene synthases which, together, produce the diterpenoid specialized metabolite sclareol in a two-step process. Labda-13-en-8-ol diphosphate synthase produces labda-13-en-8-ol diphosphate as major product from geranylgeranyl diphosphate with some minor quantities of its non-hydroxylated analogue, (9 S, 10 S)-copalyl diphosphate. Sclareol synthase then transformes these intermediates into sclareol and manool, respectively Salvia sclarea