Activating Compound | Comment | Organism | Structure |
---|---|---|---|
dithioerythritol | increased catalytic activity with 10 mM | Homo sapiens | |
additional information | NADPH is not necessary for activity | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | completely inactivates the enzyme by complexation of Mg2+ | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | - |
6-(L-erythro-1,2-dihydroxypropyl 3-triphosphate)-7,8-dihydropterin | - |
Homo sapiens | |
0.01 | - |
7,8-dihydroneopterin triphosphate | pH 7.5, 37ºC | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
19000 | - |
x * 19000, SDS-PAGE | Homo sapiens |
19000 | - |
4 * 19000, SDS-PAGE | Homo sapiens |
83000 | - |
gel filtration, seems to have 4 identical subunits | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
6-(L-erythro-1,2-dihydroxypropyl 3-triphosphate)-7,8-dihydropterin | Homo sapiens | the enzyme catalyzes the first step in the conversion of 7,8-dihydroneopterin triphosphate to tetrahydrobiopterin | 6-(1,2-dioxopropyl)-5,6,7,8-tetrahydropterin + triphosphate | - |
? | |
7,8-dihydroneopterin triphosphate | Homo sapiens | assay at | 6-pyruvoyl-5,6,7,8-tetrahydropterin + triphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, hydroxyapatite column, gel filtration, DEAE-Fractogel 650S column, SDS-PAGE | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Homo sapiens |
0.1 | - |
- |
Homo sapiens |
Storage Stability | Organism |
---|---|
-20ºC and -70ºC, Tris HCl, several months, stable | Homo sapiens |
-70°C, stable for several months | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
6-(L-erythro-1,2-dihydroxypropyl 3-triphosphate)-7,8-dihydropterin | - |
Homo sapiens | 6-(1,2-dioxopropyl)-5,6,7,8-tetrahydropterin + triphosphate | - |
? | |
6-(L-erythro-1,2-dihydroxypropyl 3-triphosphate)-7,8-dihydropterin | the enzyme catalyzes the first step in the conversion of 7,8-dihydroneopterin triphosphate to tetrahydrobiopterin | Homo sapiens | 6-(1,2-dioxopropyl)-5,6,7,8-tetrahydropterin + triphosphate | - |
? | |
7,8-dihydroneopterin triphosphate | - |
Homo sapiens | 6-pyruvoyl-5,6,7,8-tetrahydropterin + triphosphate | - |
? | |
7,8-dihydroneopterin triphosphate | assay at | Homo sapiens | 6-pyruvoyl-5,6,7,8-tetrahydropterin + triphosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 19000, SDS-PAGE | Homo sapiens |
tetramer | 4 * 19000, SDS-PAGE | Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Homo sapiens |
7.5 | - |
in Tris-HCl 100 mM | Homo sapiens |