Cloned (Comment) | Organism |
---|---|
cloned in Escherichia coli | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.012 | - |
6-(L-erythro-1,2-dihydroxypropyl 3-triphosphate)-7,8-dihydropterin | - |
Homo sapiens | |
0.0124 | - |
7,8-dihydroneopterin triphosphate | 90 KDa protein pH 7.4, 37ºC | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | necessary for activity | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
17000 | - |
x * 17000, SDS-PAGE | Homo sapiens |
17000 | - |
4 * 17000, SDS-PAGE | Homo sapiens |
90000 | - |
gel filtration | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
7,8-dihydroneopterin triphosphate | Homo sapiens | assay at | 6-pyruvoyl-5,6,7,8-tetrahydropterin + triphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q03393 | cloned in Escherichia coli | - |
Purification (Comment) | Organism |
---|---|
affinity chromatography, gel filtration | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
MOLT-4 cell | acute lymphoblastic leukemia cell line, 65% homology with rat enzyme | Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.5167 | - |
- |
Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
7,8-dihydroneopterin triphosphate | - |
Homo sapiens | 6-pyruvoyl-5,6,7,8-tetrahydropterin + triphosphate | - |
? | |
7,8-dihydroneopterin triphosphate | assay at | Homo sapiens | 6-pyruvoyl-5,6,7,8-tetrahydropterin + triphosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 17000, SDS-PAGE | Homo sapiens |
tetramer | 4 * 17000, SDS-PAGE | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | 8 | - |
Homo sapiens |