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Literature summary for 4.2.3.12 extracted from

  • Guzman, J.; Redweik, U.; Schoedon, G.; Hunziker, P.; Wiestler, O.D.; Heizmann, C.W.; Blau, N.
    Purification and characterization of 6-pyruvoyl tetrahydropterin synthase from human pituitary gland (1992), Enzyme, 46, 287-298.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
additional information NADPH is not necessary for activity Homo sapiens

General Stability

General Stability Organism
stable at all steps of purification Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
additional information L-monapterin does not inhibit activity Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.01
-
6-(L-erythro-1,2-dihydroxypropyl 3-triphosphate)-7,8-dihydropterin
-
Homo sapiens
0.01
-
7,8-dihydroneopterin triphosphate pH 7.4, 37ºC, shows positive cooperativity Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Homo sapiens
Mg2+ necessary for activity Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
16500
-
4 * 16500, SDS-PAGE Homo sapiens
16500
-
x * 16500, SDS-PAGE Homo sapiens
68000
-
gel filtration Homo sapiens
68000
-
gel filtration Salmo salar

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
7,8-dihydroneopterin triphosphate Drosophila melanogaster assay at 6-pyruvoyl-5,6,7,8-tetrahydropterin + triphosphate
-
?
7,8-dihydroneopterin triphosphate Homo sapiens assay at 6-pyruvoyl-5,6,7,8-tetrahydropterin + triphosphate
-
?
7,8-dihydroneopterin triphosphate Salmo salar assay at 6-pyruvoyl-5,6,7,8-tetrahydropterin + triphosphate
-
?

Organism

Organism UniProt Comment Textmining
Drosophila melanogaster
-
-
-
Homo sapiens
-
-
-
Salmo salar
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, DEAE-Sephadex A50 column Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
head
-
Drosophila melanogaster
-
hypophysis
-
Homo sapiens
-
liver
-
Homo sapiens
-
liver
-
Salmo salar
-
pituitary gland heat instability, it is present in the anterior pituitary endothelial cells but not in posterior pituitary Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
40
-
-
Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7,8-dihydroneopterin triphosphate
-
Drosophila melanogaster 6-pyruvoyl-5,6,7,8-tetrahydropterin + triphosphate
-
?
7,8-dihydroneopterin triphosphate
-
Homo sapiens 6-pyruvoyl-5,6,7,8-tetrahydropterin + triphosphate
-
?
7,8-dihydroneopterin triphosphate
-
Salmo salar 6-pyruvoyl-5,6,7,8-tetrahydropterin + triphosphate
-
?
7,8-dihydroneopterin triphosphate assay at Drosophila melanogaster 6-pyruvoyl-5,6,7,8-tetrahydropterin + triphosphate
-
?
7,8-dihydroneopterin triphosphate assay at Homo sapiens 6-pyruvoyl-5,6,7,8-tetrahydropterin + triphosphate
-
?
7,8-dihydroneopterin triphosphate assay at Salmo salar 6-pyruvoyl-5,6,7,8-tetrahydropterin + triphosphate
-
?

Subunits

Subunits Comment Organism
?
-
Drosophila melanogaster
?
-
Salmo salar
? x * 16500, SDS-PAGE Homo sapiens
tetramer
-
Salmo salar
tetramer 4 * 16500, SDS-PAGE Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Drosophila melanogaster
37
-
assay at Homo sapiens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
the human pituitary gland enzyme is heat instable in contrast to the enzyme from human, rat and salmon liver, and Drosophila heads Drosophila melanogaster
additional information
-
the human pituitary gland enzyme is heat instable in contrast to the enzyme from human, rat and salmon liver, and Drosophila heads Homo sapiens
additional information
-
the human pituitary gland enzyme is heat instable in contrast to the enzyme from human, rat and salmon liver, and Drosophila heads Salmo salar

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
Drosophila melanogaster
7.5
-
-
Homo sapiens
7.5
-
-
Salmo salar
7.5
-
in Tris-HCl 100 mM Homo sapiens
7.5
-
in Tris-HCl 100 mM Salmo salar

Cofactor

Cofactor Comment Organism Structure
NADPH required Homo sapiens