Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | NADPH is not necessary for activity | Homo sapiens |
General Stability | Organism |
---|---|
stable at all steps of purification | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | L-monapterin does not inhibit activity | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | - |
6-(L-erythro-1,2-dihydroxypropyl 3-triphosphate)-7,8-dihydropterin | - |
Homo sapiens | |
0.01 | - |
7,8-dihydroneopterin triphosphate | pH 7.4, 37ºC, shows positive cooperativity | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Homo sapiens | |
Mg2+ | necessary for activity | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
16500 | - |
4 * 16500, SDS-PAGE | Homo sapiens |
16500 | - |
x * 16500, SDS-PAGE | Homo sapiens |
68000 | - |
gel filtration | Homo sapiens |
68000 | - |
gel filtration | Salmo salar |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
7,8-dihydroneopterin triphosphate | Drosophila melanogaster | assay at | 6-pyruvoyl-5,6,7,8-tetrahydropterin + triphosphate | - |
? | |
7,8-dihydroneopterin triphosphate | Homo sapiens | assay at | 6-pyruvoyl-5,6,7,8-tetrahydropterin + triphosphate | - |
? | |
7,8-dihydroneopterin triphosphate | Salmo salar | assay at | 6-pyruvoyl-5,6,7,8-tetrahydropterin + triphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Drosophila melanogaster | - |
- |
- |
Homo sapiens | - |
- |
- |
Salmo salar | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, DEAE-Sephadex A50 column | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
head | - |
Drosophila melanogaster | - |
hypophysis | - |
Homo sapiens | - |
liver | - |
Homo sapiens | - |
liver | - |
Salmo salar | - |
pituitary gland | heat instability, it is present in the anterior pituitary endothelial cells but not in posterior pituitary | Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
7,8-dihydroneopterin triphosphate | - |
Drosophila melanogaster | 6-pyruvoyl-5,6,7,8-tetrahydropterin + triphosphate | - |
? | |
7,8-dihydroneopterin triphosphate | - |
Homo sapiens | 6-pyruvoyl-5,6,7,8-tetrahydropterin + triphosphate | - |
? | |
7,8-dihydroneopterin triphosphate | - |
Salmo salar | 6-pyruvoyl-5,6,7,8-tetrahydropterin + triphosphate | - |
? | |
7,8-dihydroneopterin triphosphate | assay at | Drosophila melanogaster | 6-pyruvoyl-5,6,7,8-tetrahydropterin + triphosphate | - |
? | |
7,8-dihydroneopterin triphosphate | assay at | Homo sapiens | 6-pyruvoyl-5,6,7,8-tetrahydropterin + triphosphate | - |
? | |
7,8-dihydroneopterin triphosphate | assay at | Salmo salar | 6-pyruvoyl-5,6,7,8-tetrahydropterin + triphosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | - |
Drosophila melanogaster |
? | - |
Salmo salar |
? | x * 16500, SDS-PAGE | Homo sapiens |
tetramer | - |
Salmo salar |
tetramer | 4 * 16500, SDS-PAGE | Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Drosophila melanogaster |
37 | - |
assay at | Homo sapiens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
the human pituitary gland enzyme is heat instable in contrast to the enzyme from human, rat and salmon liver, and Drosophila heads | Drosophila melanogaster |
additional information | - |
the human pituitary gland enzyme is heat instable in contrast to the enzyme from human, rat and salmon liver, and Drosophila heads | Homo sapiens |
additional information | - |
the human pituitary gland enzyme is heat instable in contrast to the enzyme from human, rat and salmon liver, and Drosophila heads | Salmo salar |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Drosophila melanogaster |
7.5 | - |
- |
Homo sapiens |
7.5 | - |
- |
Salmo salar |
7.5 | - |
in Tris-HCl 100 mM | Homo sapiens |
7.5 | - |
in Tris-HCl 100 mM | Salmo salar |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | required | Homo sapiens |