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Literature summary for 4.2.1.79 extracted from
- Oxidation of propionate to pyruvate in Escherichia coli: Involvement of methylcitrate dehydratase and aconitase (2002), Eur. J. Biochem., 269, 6184-6194.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | gene prpD, part of the prp operon, induction and expression depends on propionate during growth | Escherichia coli |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| genomic structure of prp operon, expression studies in Escherichia coli K12 strain W3350, and overexpression of the N-terminally His-tagged enzyme | Escherichia coli |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.44 | - |
(2S,3S)-2-methylcitrate | - |
Escherichia coli |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 54000 | - |
gel filtration | Escherichia coli |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (2S,3S)-hydroxybutane-1,2,3-tricarboxylate | Escherichia coli | enzyme performs the isomerization of (2S,3S)-methylcitrate to (2R,3S)-2-methylisocitrate together with aconitase, both enzymes are essential, overview | (Z)-but-2-ene-1,2,3-tricarboxylate + H2O | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | P77243 | gene prpD, part of the prp operon, induction and expression depends on propionate during growth | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged enzyme from overexpressing strain | Escherichia coli |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| culture condition:propionate-grown cell | - |
Escherichia coli | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (2S,3S)-2-methylcitrate | - |
Escherichia coli | cis-2-methylaconitate + H2O | - |
? | |
| (2S,3S)-hydroxybutane-1,2,3-tricarboxylate | i.e. 2-methylcitrate, highly specific for, stereospecific for (2S,3S)-methylcitrate, the reverse reaction is an unusual syn-elimination | Escherichia coli | (Z)-but-2-ene-1,2,3-tricarboxylate + H2O | i.e. 2-methyl-cis-aconitate | ? | |
| (2S,3S)-hydroxybutane-1,2,3-tricarboxylate | enzyme performs the isomerization of (2S,3S)-methylcitrate to (2R,3S)-2-methylisocitrate together with aconitase, both enzymes are essential, overview | Escherichia coli | (Z)-but-2-ene-1,2,3-tricarboxylate + H2O | - |
r | |
| cis-aconitate | 5fold lower activity than with 2-methylcitrate | Escherichia coli | ? | - |
? | |
| additional information | (2R,3S)-isocitrate and citrate are poor substrates, no activity with trans-aconitate, threo-2-methylisocitrate, erythro-2-methylisocitrate, (S)-malate and (R)-malate | Escherichia coli | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| methylcitrate dehydratase | - |
Escherichia coli |
| PrpD | - |
Escherichia coli |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Escherichia coli |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Escherichia coli | isoelectric focusing | - |
5.7 |
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