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Literature summary for 4.2.1.70 extracted from

  • Preumont, A.; Snoussi, K.; Stroobant, V.; Collet, J.F.; Van Schaftingen, E.
    Molecular identification of pseudouridine-metabolizing enzymes (2008), J. Biol. Chem., 283, 25238-25246.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression as His-tagged enzyme in Escherichia coli Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
Ni2+
-
Escherichia coli
Zn2+
-
Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.06
-
pseudouridine 5'-phosphate pH 7.1, 30°C Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ high activity Escherichia coli
Fe2+ high activity Escherichia coli
Mn2+ high activity Escherichia coli
additional information YeiN is dependent on divalent cations Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
uracil + D-ribose 5-phosphate Thermotoga maritima the association of IndA with pseudouridine kinase indicates that it serves physiologically to metabolize pseudouridine 5'-phosphate rather than to form it pseudouridine 5'-phosphate + H2O
-
r
uracil + D-ribose 5-phosphate Escherichia coli the enzyme is involved in the metabolism of pseudouridine in uropathogenic Escherichia coli pseudouridine 5'-phosphate + H2O
-
r

Organism

Organism UniProt Comment Textmining
Escherichia coli P33025 gene yeiN
-
Thermotoga maritima
-
gene indA
-

Purification (Commentary)

Purification (Comment) Organism
-
Thermotoga maritima
recombinant His-tagged enzyme from Escherichia coli by affinity chromatography Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information activity measurement in a two-step assay Thermotoga maritima ?
-
?
additional information measuring the production of ribose 5-phosphate with a ribose-5-phosphate isomerase/ribulose-5-phosphate reductase-coupled assay, no activity with ribonucleotide monophosphates AMP, UMP, GMP, and CMP, overview Escherichia coli ?
-
?
uracil + D-ribose 5-phosphate
-
Escherichia coli pseudouridine 5'-phosphate + H2O
-
r
uracil + D-ribose 5-phosphate the association of IndA with pseudouridine kinase indicates that it serves physiologically to metabolize pseudouridine 5'-phosphate rather than to form it Thermotoga maritima pseudouridine 5'-phosphate + H2O
-
r
uracil + D-ribose 5-phosphate the enzyme is involved in the metabolism of pseudouridine in uropathogenic Escherichia coli Escherichia coli pseudouridine 5'-phosphate + H2O
-
r
uracil + D-ribose 5-phosphate IndA catalyzes the conversion of pseudouridine 5'-phosphate to uracil and ribose 5-phosphate in a divalent cation-dependent manner by cleavage of the glycosidic C-C bond of pseudouridine Thermotoga maritima pseudouridine 5'-phosphate + H2O
-
r

Synonyms

Synonyms Comment Organism
IndA
-
Thermotoga maritima
pseudouridine-5'-phosphate glycosidase
-
Thermotoga maritima
pseudouridine-5'-phosphate glycosidase
-
Escherichia coli
YeiN
-
Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at, both reaction directions Thermotoga maritima
30
-
assay at, both reaction directions Escherichia coli

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
no activity at or below Thermotoga maritima

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.1
-
assay at, both reaction directions Thermotoga maritima
7.1
-
assay at, both reaction directions Escherichia coli