Cloned (Comment) | Organism |
---|---|
expression as His-tagged enzyme in Escherichia coli | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ni2+ | - |
Escherichia coli | |
Zn2+ | - |
Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.06 | - |
pseudouridine 5'-phosphate | pH 7.1, 30°C | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | high activity | Escherichia coli | |
Fe2+ | high activity | Escherichia coli | |
Mn2+ | high activity | Escherichia coli | |
additional information | YeiN is dependent on divalent cations | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
uracil + D-ribose 5-phosphate | Thermotoga maritima | the association of IndA with pseudouridine kinase indicates that it serves physiologically to metabolize pseudouridine 5'-phosphate rather than to form it | pseudouridine 5'-phosphate + H2O | - |
r | |
uracil + D-ribose 5-phosphate | Escherichia coli | the enzyme is involved in the metabolism of pseudouridine in uropathogenic Escherichia coli | pseudouridine 5'-phosphate + H2O | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P33025 | gene yeiN | - |
Thermotoga maritima | - |
gene indA | - |
Purification (Comment) | Organism |
---|---|
- |
Thermotoga maritima |
recombinant His-tagged enzyme from Escherichia coli by affinity chromatography | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | activity measurement in a two-step assay | Thermotoga maritima | ? | - |
? | |
additional information | measuring the production of ribose 5-phosphate with a ribose-5-phosphate isomerase/ribulose-5-phosphate reductase-coupled assay, no activity with ribonucleotide monophosphates AMP, UMP, GMP, and CMP, overview | Escherichia coli | ? | - |
? | |
uracil + D-ribose 5-phosphate | - |
Escherichia coli | pseudouridine 5'-phosphate + H2O | - |
r | |
uracil + D-ribose 5-phosphate | the association of IndA with pseudouridine kinase indicates that it serves physiologically to metabolize pseudouridine 5'-phosphate rather than to form it | Thermotoga maritima | pseudouridine 5'-phosphate + H2O | - |
r | |
uracil + D-ribose 5-phosphate | the enzyme is involved in the metabolism of pseudouridine in uropathogenic Escherichia coli | Escherichia coli | pseudouridine 5'-phosphate + H2O | - |
r | |
uracil + D-ribose 5-phosphate | IndA catalyzes the conversion of pseudouridine 5'-phosphate to uracil and ribose 5-phosphate in a divalent cation-dependent manner by cleavage of the glycosidic C-C bond of pseudouridine | Thermotoga maritima | pseudouridine 5'-phosphate + H2O | - |
r |
Synonyms | Comment | Organism |
---|---|---|
IndA | - |
Thermotoga maritima |
pseudouridine-5'-phosphate glycosidase | - |
Thermotoga maritima |
pseudouridine-5'-phosphate glycosidase | - |
Escherichia coli |
YeiN | - |
Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at, both reaction directions | Thermotoga maritima |
30 | - |
assay at, both reaction directions | Escherichia coli |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
no activity at or below | Thermotoga maritima |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.1 | - |
assay at, both reaction directions | Thermotoga maritima |
7.1 | - |
assay at, both reaction directions | Escherichia coli |