KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00042 | - |
tRNAVal | Pus1, yeast tRNAVal | Saccharomyces cerevisiae | |
0.00074 | - |
tRNAIle | Pus1, intronless yeast tRNAIle | Saccharomyces cerevisiae | |
0.0015 | - |
tRNAVal | Pus1, yeast tRNAVal, in the presence of 5 mM EDTA and in the absence of Mg2+ | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Saccharomyces cerevisiae | pseudouridine synthase Pus1p displays a multisite substrate specificity | ? | - |
? | |
additional information | Saccharomyces cerevisiae | a model for Pus1 mechanism | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | pseudouridine synthase Pus1p displays a multisite substrate specificity | Saccharomyces cerevisiae | ? | - |
? | |
additional information | a model for Pus1 mechanism | Saccharomyces cerevisiae | ? | - |
? | |
tRNAIle | - |
Saccharomyces cerevisiae | ? | - |
? | |
tRNAVal | - |
Saccharomyces cerevisiae | ? | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00667 | - |
tRNAVal | substrate from yeast, limiting | Saccharomyces cerevisiae | |
0.00833 | - |
tRNAVal | substrate from yeast, in absence of Mg2+ | Saccharomyces cerevisiae | |
0.00833 | - |
tRNAIle | substrate from yeast, intronless, limiting | Saccharomyces cerevisiae |