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Literature summary for 4.2.1.70 extracted from

  • Arena, F.; Ciliberto, G.; Ciampi, S.; Cortese, R.
    Purification of pseudouridylate synthetase I from Salmonella typhimurium (1978), Nucleic Acids Res., 5, 4523-4536.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.2.1.70

General Stability

General Stability Organism
mercaptoethanol is very effective in restoring the original activity of aged enzyme preparations Salmonella enterica subsp. enterica serovar Typhimurium

Inhibitors

Inhibitors Comment Organism Structure
iodoacetamide 150 mM, in about 5 min all activity is lost Salmonella enterica subsp. enterica serovar Typhimurium
tRNA wild-type RNA, 50% inhibition at a concentration of 0.005 mM Salmonella enterica subsp. enterica serovar Typhimurium

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000
-
 2 * 50000, glycerol gradient sedimentation, tRNA induced dimerization Salmonella enterica subsp. enterica serovar Typhimurium

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Salmonella enterica subsp. enterica serovar Typhimurium pseudouridylate synthetase I is responsible for the biosynthesis of pseudouridine in the anticodon region of several tRNA species ?
-
 ?

Organism

Organism UniProt Comment Textmining
Salmonella enterica subsp. enterica serovar Typhimurium
-
 TA265 (wild-type strain of LT-2)
-
Salmonella enterica subsp. enterica serovar Typhimurium
-
 TA253 (carrying the hisT 1504 mutation)
-

Purification (Commentary)

Purification (Comment) Organism
pseudouridylate synthetase I Salmonella enterica subsp. enterica serovar Typhimurium

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Salmonella enterica subsp. enterica serovar Typhimurium

Storage Stability

Storage Stability Organism
4°C, storage for 40 days, preincubation with 15 mM mercaptoethanol restores the original activity Salmonella enterica subsp. enterica serovar Typhimurium

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information pseudouridylate synthetase I is responsible for the biosynthesis of pseudouridine in the anticodon region of several tRNA species Salmonella enterica subsp. enterica serovar Typhimurium ?
-
 ?
uracil + D-ribose 5-phosphate
-
 Salmonella enterica subsp. enterica serovar Typhimurium pseudouridine 5'-phosphate + H2O
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 50000, glycerol gradient sedimentation, tRNA induced dimerization Salmonella enterica subsp. enterica serovar Typhimurium
monomer 1* 50000 glycerol gradient sedimentation Salmonella enterica subsp. enterica serovar Typhimurium

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.005
-
 tRNA wild-type RNA Salmonella enterica subsp. enterica serovar Typhimurium