Application | Comment | Organism |
---|---|---|
industry | overexpression of pheAfbr and/or wild-type aroF is helpful for the enhancement of the key enzymes in L-phenylalanine biosynthesis, which is an important prerequisite for improved production of L-phenylalanine with engineered strains | Escherichia coli |
Cloned (Comment) | Organism |
---|---|
expressed in different engineered Escherichia coli WSH-Z06 strains (pAP-B, pAP-B01, pAP-B02 and pAP-B03) | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
T326P | mutant harboring pheAfbr retains more than 70% of CM and PDT activities even in the presence of 200 mM L-phenylalanine, has potential in overproduction of L-phenylalanine | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
L-phenylalanine | feedback inhibition. PDT of Escherichia coli WSH-Z06 is almost completely inhibited by 10 mM L-phenylalanine (92.9% activity loss). PDT of Escherichia coli WSH-Z06 (pAP-B03) exhibits strong resistance to 200 mM L-phenylalanine, manifested by the high residual activities (10 mM L-phenylalanine with only 7.1% loss of activity) | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
43100 | - |
x * 43100, SDS-PAGE | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | C7EXK8 | the enzyme possesses prephenate dehydratase (EC 4.2.1.51) and chorismate mutase activity (EC 5.4.99.5); K06 | - |
Escherichia coli K06 | C7EXK8 | the enzyme possesses prephenate dehydratase (EC 4.2.1.51) and chorismate mutase activity (EC 5.4.99.5); K06 | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
3100 | - |
strain WSH-Z06 (pAP-B) | Escherichia coli |
18000 | - |
strain WSH-Z06 (pAP-B03) | Escherichia coli |
18600 | - |
strain WSH-Z06 (pAP-B01) | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
prephenate | - |
Escherichia coli | phenylpyruvate + H2O + CO2 | - |
? | |
prephenate | - |
Escherichia coli K06 | phenylpyruvate + H2O + CO2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 43100, SDS-PAGE | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
chorismate mutase-prephenate dehydratase | the enzyme also possesses chorismate mutase activity (EC 5.4.99.5) | Escherichia coli |
CMPDT | the enzyme also possesses chorismate mutase activity (EC 5.4.99.5) | Escherichia coli |
PheA | - |
Escherichia coli |
General Information | Comment | Organism |
---|---|---|
malfunction | use of a T326P mutation in the regulation domain of pheA (pheAfbr) for L-phenylalanine production. Overexpression of pheAfbr and/or wild-type aroF is helpful for the enhancement of the key enzymes in L-phenylalanine biosynthesis | Escherichia coli |