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Literature summary for 4.2.1.51 extracted from

  • Yamada, T.; Matsuda, F.; Kasai, K.; Fukuoka, S.; Kitamura, K.; Tozawa, Y.; Miyagawa, H.; Wakasa, K.
    Mutation of a rice gene encoding a phenylalanine biosynthetic enzyme results in accumulation of phenylalanine and tryptophan (2008), Plant Cell, 20, 1316-1329.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
-
Oryza sativa
expressed in Escherichia coli, mtr1-D mutant gene, callus transformation by Agrobacterium tumefaciens Oryza sativa

Protein Variants

Protein Variants Comment Organism
S298I mutant Mtr1 Oryza sativa

Inhibitors

Inhibitors Comment Organism Structure
L-phenylalanine feedback regulation wild-type, mutant enzyme MTR1 (PDT S298I) shows a reduced feedback sensitivity, resulting in phenylalanine accumulation; inhibits activity of wild-type enzyme, mutant enzyme shows reduced feedback sensitivity, accumulation of L-phenylalanine Oryza sativa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.12
-
L-arogenate wild-type, rice PDT is a bifunctional enzyme Oryza sativa
0.17
-
L-arogenate mutant S298I, rice PDT is a bifunctional enzyme Oryza sativa
2.29
-
phenylpyruvate wild-type Oryza sativa
2.29
-
prephenate wild-type, rice PDT is a bifunctional enzyme Oryza sativa
4.88
-
phenylpyruvate Mtr1 mutant Oryza sativa
4.88
-
prephenate mutant S298I, rice PDT is a bifunctional enzyme Oryza sativa

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast
-
Oryza sativa 9507
-
chloroplast targeting sequence, in vitro import assay Oryza sativa 9507
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35000
-
processed to a product of 35000 Dalton during incubation with pea chloroplasts, protected from degradation by thermolysin, organellar targeting sequence Oryza sativa
40000
-
wild-type, SDS-PAGE Oryza sativa

Organism

Organism UniProt Comment Textmining
Oryza sativa A8CF65 Oryza sativa subsp. japonica (Rice)
-
Oryza sativa Q6Z3Y3 wild-type and Mtr1 mutant
-

Purification (Commentary)

Purification (Comment) Organism
gel filtration, SDS-PAGE Oryza sativa

Source Tissue

Source Tissue Comment Organism Textmining
callus wild-type, and transformed callus lines expressing mtr1-D mutation, experimental system for feeding experiments with external L-phenylalanine Oryza sativa
-
seedling feeding experiments with external L-phenylalanine, accumulation of L-trytophan Oryza sativa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
Mtr1 mutant of rice indicates accumulation of L-phenylalanine, L-trytophan, and of several phenylpropanoids, suggesting a link between the synthesis of L-phenylalanine and L-trytophan, mtr1-D mutant gene indicates point mutation in the putative allosteric regulatory region, wild-type enzyme is feedback regulated by L-phenylalanine, Mtr1 mutant has reduced feedback activity and accumulates L-phenylalanine, critical for regulating the size of the pool of L-phenylalanine in plant cells Oryza sativa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-arogenate
-
Oryza sativa L-phenylalanine + CO2 + H2O
-
?
prephenate bifunctional enzyme, PDT activity with prephenate and ADT activity with arogenate Oryza sativa phenylpyruvate + H2O + CO2
-
?
prephenate bifunctional enzyme, prephenate dehydratase-arogenate dehydratase, preference for arogenate as substrate indicates primary function as an arogenate dehydratase (ADT) Oryza sativa phenylpyruvate + H2O + CO2
-
?

Synonyms

Synonyms Comment Organism
PDT bifunctional enzyme, PDT activity with prephenate and ADT activity with arogenate Oryza sativa
PDT protein
-
Oryza sativa
prephenate dehydratase bifunctional enzyme, PDT activity with prephenate and ADT activity with arogenate Oryza sativa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
spectrophotometric activity assay at Oryza sativa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.2
-
spectrophotometric activity assay at Oryza sativa

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
7.36
-
L-phenylalanine wild-type, rice PDT is a bifunctional enzyme Oryza sativa
61.5
-
L-phenylalanine mutant enzyme S298I, rice PDT is a bifunctional enzyme Oryza sativa

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
additional information
-
L-phenylalanine more than 0.5 Oryza sativa additional information
0.016
-
wild-type, rice PDT is a bifunctional enzyme Oryza sativa L-phenylalanine