Cloned (Comment) | Organism |
---|---|
- |
Oryza sativa |
expressed in Escherichia coli, mtr1-D mutant gene, callus transformation by Agrobacterium tumefaciens | Oryza sativa |
Protein Variants | Comment | Organism |
---|---|---|
S298I | mutant Mtr1 | Oryza sativa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
L-phenylalanine | feedback regulation wild-type, mutant enzyme MTR1 (PDT S298I) shows a reduced feedback sensitivity, resulting in phenylalanine accumulation; inhibits activity of wild-type enzyme, mutant enzyme shows reduced feedback sensitivity, accumulation of L-phenylalanine | Oryza sativa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.12 | - |
L-arogenate | wild-type, rice PDT is a bifunctional enzyme | Oryza sativa | |
0.17 | - |
L-arogenate | mutant S298I, rice PDT is a bifunctional enzyme | Oryza sativa | |
2.29 | - |
phenylpyruvate | wild-type | Oryza sativa | |
2.29 | - |
prephenate | wild-type, rice PDT is a bifunctional enzyme | Oryza sativa | |
4.88 | - |
phenylpyruvate | Mtr1 mutant | Oryza sativa | |
4.88 | - |
prephenate | mutant S298I, rice PDT is a bifunctional enzyme | Oryza sativa |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | - |
Oryza sativa | 9507 | - |
chloroplast | targeting sequence, in vitro import assay | Oryza sativa | 9507 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35000 | - |
processed to a product of 35000 Dalton during incubation with pea chloroplasts, protected from degradation by thermolysin, organellar targeting sequence | Oryza sativa |
40000 | - |
wild-type, SDS-PAGE | Oryza sativa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oryza sativa | A8CF65 | Oryza sativa subsp. japonica (Rice) | - |
Oryza sativa | Q6Z3Y3 | wild-type and Mtr1 mutant | - |
Purification (Comment) | Organism |
---|---|
gel filtration, SDS-PAGE | Oryza sativa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
callus | wild-type, and transformed callus lines expressing mtr1-D mutation, experimental system for feeding experiments with external L-phenylalanine | Oryza sativa | - |
seedling | feeding experiments with external L-phenylalanine, accumulation of L-trytophan | Oryza sativa | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
Mtr1 mutant of rice indicates accumulation of L-phenylalanine, L-trytophan, and of several phenylpropanoids, suggesting a link between the synthesis of L-phenylalanine and L-trytophan, mtr1-D mutant gene indicates point mutation in the putative allosteric regulatory region, wild-type enzyme is feedback regulated by L-phenylalanine, Mtr1 mutant has reduced feedback activity and accumulates L-phenylalanine, critical for regulating the size of the pool of L-phenylalanine in plant cells | Oryza sativa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-arogenate | - |
Oryza sativa | L-phenylalanine + CO2 + H2O | - |
? | |
prephenate | bifunctional enzyme, PDT activity with prephenate and ADT activity with arogenate | Oryza sativa | phenylpyruvate + H2O + CO2 | - |
? | |
prephenate | bifunctional enzyme, prephenate dehydratase-arogenate dehydratase, preference for arogenate as substrate indicates primary function as an arogenate dehydratase (ADT) | Oryza sativa | phenylpyruvate + H2O + CO2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PDT | bifunctional enzyme, PDT activity with prephenate and ADT activity with arogenate | Oryza sativa |
PDT protein | - |
Oryza sativa |
prephenate dehydratase | bifunctional enzyme, PDT activity with prephenate and ADT activity with arogenate | Oryza sativa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
spectrophotometric activity assay at | Oryza sativa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.2 | - |
spectrophotometric activity assay at | Oryza sativa |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
7.36 | - |
L-phenylalanine | wild-type, rice PDT is a bifunctional enzyme | Oryza sativa | |
61.5 | - |
L-phenylalanine | mutant enzyme S298I, rice PDT is a bifunctional enzyme | Oryza sativa |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
additional information | - |
L-phenylalanine more than 0.5 | Oryza sativa | additional information | |
0.016 | - |
wild-type, rice PDT is a bifunctional enzyme | Oryza sativa | L-phenylalanine |