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Literature summary for 4.2.1.47 extracted from
- Differential role of NADP+ and NADPH in the activity and structure of GDP-D-mannose 4,6-dehydratase from two Chlorella viruses (2008), J. Biol. Chem., 283, 184-193.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli as glutathione S-transferase fusion protein | Paramecium bursaria Chlorella virus-1 |
| expressed in Escherichia coli as glutathione S-transferase fusion protein | Acanthocystis turfacea chlorella virus 1 |
| expression in Escherichia coli | Paramecium bursaria Chlorella virus-1 |
| expression in Escherichia coli | Acanthocystis turfacea chlorella virus 1 |
General Stability
| General Stability | Organism |
|---|---|
| NADPH is essential for stabilization and function of the enzyme | Paramecium bursaria Chlorella virus-1 |
| NADPH is essential for stabilization and function of the enzyme | Acanthocystis turfacea chlorella virus 1 |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| NADP+ | conversion of NADPH to NADP+ results in complete inactivation of the enzyme | Acanthocystis turfacea chlorella virus 1 |  |
| NADP+ | conversion of NADPH to NADP+ results in complete inactivation of the enzyme | Paramecium bursaria Chlorella virus-1 | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 87000 | - |
gel filtration | Paramecium bursaria Chlorella virus-1 |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Acanthocystis turfacea chlorella virus 1 | - |
- |
- |
| Paramecium bursaria Chlorella virus-1 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Paramecium bursaria Chlorella virus-1 |
- |
Acanthocystis turfacea chlorella virus 1 |
| GSH-Sepharose 4B column chromatography | Paramecium bursaria Chlorella virus-1 |
| GSH-Sepharose 4B column chromatography | Acanthocystis turfacea chlorella virus 1 |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.48 | - |
- |
Paramecium bursaria Chlorella virus-1 |
| 0.63 | - |
- |
Acanthocystis turfacea chlorella virus 1 |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| GDP-D-mannose | - |
Paramecium bursaria Chlorella virus-1 | GDP-4-dehydro-6-deoxy-D-mannose + H2O | - |
? | |
| GDP-D-mannose | - |
Acanthocystis turfacea chlorella virus 1 | GDP-4-dehydro-6-deoxy-D-mannose + H2O | - |
? | |
| GDP-mannose | ATCV-1GMD has no reductase activity | Paramecium bursaria Chlorella virus-1 | GDP-4-dehydro-6-deoxy-D-mannose + H2O | - |
? | |
| GDP-mannose | ATCV-1GMD has no reductase activity | Acanthocystis turfacea chlorella virus 1 | GDP-4-dehydro-6-deoxy-D-mannose + H2O | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | NADPH is essential for maintaining the oligomerization status | Paramecium bursaria Chlorella virus-1 |
| More | NADPH is essential for maintaining the oligomerization status | Acanthocystis turfacea chlorella virus 1 |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ATCV-1GMD | - |
Acanthocystis turfacea chlorella virus 1 |
| GDP-D-mannose 4,6-dehydratase | - |
Paramecium bursaria Chlorella virus-1 |
| GDP-D-mannose 4,6-dehydratase | - |
Acanthocystis turfacea chlorella virus 1 |
| GMD | - |
Paramecium bursaria Chlorella virus-1 |
| GMD | - |
Acanthocystis turfacea chlorella virus 1 |
| PBCV-1 GMD | - |
Paramecium bursaria Chlorella virus-1 |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| additional information | the enzyme has a very low affinity for NADP+ | Paramecium bursaria Chlorella virus-1 | |
| additional information | the enzyme has a very low affinity for NADP+ | Acanthocystis turfacea chlorella virus 1 | |
| NADPH | dependent on | Paramecium bursaria Chlorella virus-1 | |
| NADPH | dependent on | Acanthocystis turfacea chlorella virus 1 | |
| NADPH | the enzymatically active proteins contain tightly bound NADPH that essential for maintaining the oligomerization status as well as for the stabilization and function of the enzyme | Paramecium bursaria Chlorella virus-1 | |
| NADPH | the enzymatically active proteins contain tightly bound NADPH that is essential for maintaining the oligomerization status as well as for the stabilization and function of the enzyme | Acanthocystis turfacea chlorella virus 1 | |
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