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Literature summary for 4.2.1.47 extracted from

  • Somoza, J.R.; Menon, S.; Schmidt, H.; Joseph-McCarthy, D.; Dessen, A.; Stahl, M.L.; Somers, W.S.; Sullivan, F.X.
    Structural and kinetic analysis of Escherichia coli GDP-mannose 4,6 dehydratase provides insights into the enzyme's catalytic mechanism and regulation by GDP-fucose (2000), Structure Fold. Des., 8, 123-135.
    View publication on PubMed
Search for more literature for 4.2.1.47

Cloned(Commentary)

Cloned (Comment) Organism
-
 Escherichia coli

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion method Escherichia coli

Protein Variants

Protein Variants Comment Organism
K161A 3000fold reduced kcat Escherichia coli
T133V 3000fold reduced kcat Escherichia coli
Y157F 3000fold reduced kcat Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
GDP-fucose competitive inhibition Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.11
-
 GDP-mannose pH 7, 37°C, T133V mutant Escherichia coli
0.28
-
 GDP-mannose pH 7, 37°C, wild-type Escherichia coli
0.501
-
 GDP-mannose pH 7, 37°C, K161A mutant Escherichia coli
1.02
-
 GDP-mannose pH 7, 37°C, Y157F mutant Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Escherichia coli

Reaction

Reaction Comment Organism Reaction ID
GDP-alpha-D-mannose = GDP-4-dehydro-alpha-D-rhamnose + H2O mechanism Escherichia coli
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GDP-mannose
-
 Escherichia coli GDP-4-dehydro-6-deoxy-D-mannose + H2O
-
 ?

Subunits

Subunits Comment Organism
dimer homodimer, gel filtration, equilibrium ultracentrifugation experiments Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0015
-
 GDP-mannose pH 7, 37°C, T133V mutant Escherichia coli
0.0019
-
 GDP-mannose pH 7, 37°C, Y157F mutant Escherichia coli
0.0053
-
 GDP-mannose pH 7, 37°C, K161A mutant Escherichia coli
5
-
 GDP-mannose pH 7, 37°C, wild-type Escherichia coli

Cofactor

Cofactor Comment Organism Structure
NADP+ one NADP+ per monomer Escherichia coli

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0789
-
 GDP-fucose pH 7, 37°C Escherichia coli