Crystallization (Comment) | Organism |
---|---|
hanging drop vapor diffusion method | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
D366A | reduced kcat for D-glucarate and L-idarate | Escherichia coli |
D366N | reduced kcat for D-glucarate and 3fold reduced kcat for L-idarate | Escherichia coli |
H339A | reduced kcat for D-glucarate and L-idarate | Escherichia coli |
H339N | reduced kcat for D-glucarate and L-idarate | Escherichia coli |
H339Q | reduced kcat for D-glucarate and L-idarate | Escherichia coli |
K207Q | reduced kcat for D-glucarate and L-idarate | Escherichia coli |
K207R | reduced kcat for D-glucarate and L-idarate | Escherichia coli |
Y150F | significant reduction of kcat for D-glucarate and L-idarate | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-deoxy-D-glucarate | - |
Escherichia coli | |
xylarohydroxamate | - |
Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | located at the active site | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P0AES2 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-deoxy-4-fluoro-D-glucarate | - |
Escherichia coli | ? | - |
? | |
D-glucarate | - |
Escherichia coli | 3-deoxy-L-threo-2-hexulosarate + H2O | - |
? | |
L-idarate | - |
Escherichia coli | 3-deoxy-L-threo-2-hexulosarate | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00025 | - |
L-idarate | H339N mutant, pH 7.5, 22°C | Escherichia coli | |
0.00033 | - |
D-glucarate | H339N mutant, pH 7.5, 22°C | Escherichia coli | |
0.001 | - |
D-glucarate | K207R mutant, pH 7.5, 22°C | Escherichia coli | |
0.0015 | - |
L-idarate | K207Q mutant, pH 7.5, 22°C | Escherichia coli | |
0.0015 | - |
L-idarate | K207R mutant, pH 7.5, 22°C | Escherichia coli | |
0.005 | - |
D-glucarate | K207Q mutant, pH 7.5, 22°C | Escherichia coli | |
0.0051 | - |
L-idarate | H339A mutant, pH 7.5, 22°C | Escherichia coli | |
0.016 | - |
D-glucarate | H339A mutant, pH 7.5, 22°C | Escherichia coli | |
0.02 | - |
D-glucarate | H339Q mutant, pH 7.5, 22°C | Escherichia coli | |
0.02 | - |
L-idarate | H339Q mutant, pH 7.5, 22°C | Escherichia coli | |
0.08 | - |
L-idarate | Y150F mutant, pH 7.5, 22°C | Escherichia coli | |
0.19 | - |
D-glucarate | Y150F mutant, pH 7.5, 22°C | Escherichia coli | |
1 | - |
D-glucarate | D366A mutant, pH 7.5, 22°C | Escherichia coli | |
1.5 | - |
L-idarate | D366A mutant, pH 7.5, 22°C | Escherichia coli | |
1.8 | - |
D-glucarate | D366N mutant, pH 7.5, 22°C | Escherichia coli | |
8 | - |
L-idarate | D366N mutant, pH 7.5, 22°C | Escherichia coli | |
34 | - |
L-idarate | pH 7.5, 22°C, wild-type enzyme | Escherichia coli | |
35 | - |
D-glucarate | pH 7.5, 22°C, wild-type enzyme | Escherichia coli |