Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | several metabolites tested, but without effect | Clostridium pasteurianum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | - |
Clostridium pasteurianum | |
dithiothreitol | - |
Clostridium pasteurianum | |
EDTA | - |
Clostridium pasteurianum | |
L-cysteine | - |
Clostridium pasteurianum | |
p-chloromercuribenzoate | - |
Clostridium pasteurianum | |
sulfhydryl compounds | - |
Clostridium pasteurianum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
5.5 | - |
D-gluconate | - |
Clostridium pasteurianum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | Fe2+ best activator, Mn2+, Co2+, Mg2+ can partially replace | Clostridium pasteurianum | |
Fe2+ | - |
Clostridium pasteurianum | |
Mg2+ | Fe2+ best activator, Mn2+, Co2+, Mg2+ can partially replace | Clostridium pasteurianum | |
Mn2+ | Fe2+ most effective, Mn2+, Co2+, Mg2+ can partially replace | Clostridium pasteurianum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
64000 | - |
2 * 64000, SDS-PAGE | Clostridium pasteurianum |
118000 | - |
sucrose density gradient centrifugation | Clostridium pasteurianum |
131000 | - |
sedimentation equilibrium, gel filtration | Clostridium pasteurianum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-gluconate | Clostridium pasteurianum | first enzyme in modified Entner-Doudoroff pathway | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Clostridium pasteurianum | - |
- |
- |
Oxidation Stability | Organism |
---|---|
inactivation by O2, activity restored by sulfhydryl compounds in the presence of Fe2+ | Clostridium pasteurianum |
Purification (Comment) | Organism |
---|---|
- |
Clostridium pasteurianum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
132 | - |
- |
Clostridium pasteurianum |
Storage Stability | Organism |
---|---|
-20°C, Tris-HCl buffer, 50% glycerol, 30% loss of activity within 5 months | Clostridium pasteurianum |
4°C, Tris-HCl buffer, pH 7.1, reactivated enzyme, at least 3 weeks under anaerobic conditions | Clostridium pasteurianum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-gluconate | - |
Clostridium pasteurianum | 2-dehydro-3-deoxy-D-gluconate | - |
? | |
D-gluconate | first enzyme in modified Entner-Doudoroff pathway | Clostridium pasteurianum | ? | - |
? | |
additional information | only substrate is D-gluconate, not: D-galactonate, D-galactarate, D-galacturonate, 6-phospho-D-gluconate, D-arabonate, D-xylonate, L-mannonate, D-glucoheptonate | Clostridium pasteurianum | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 64000, SDS-PAGE | Clostridium pasteurianum |
More | number of sulfhydryl groups | Clostridium pasteurianum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
enzyme assay at | Clostridium pasteurianum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
reactivation possible after 45 min exposure which leads to complete loss of activity, higher temperatures cause irreversible heat inactivation | Clostridium pasteurianum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.3 | 7.8 | - |
Clostridium pasteurianum |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | - |
Clostridium pasteurianum |