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Literature summary for 4.2.1.36 extracted from
- Structural characterization and comparison of the large subunits of IPM isomerase and homoaconitase from Methanococcus jannaschii (2014), Acta Crystallogr. Sect. D, 70, 922-931.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) Star cells | Methanocaldococcus jannaschii |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| sitting drop vapor diffusion method, small subunit, using 60% (w/v) 2-methyl-2,4-pentanediol and 0.1 M bicine pH 9.7 | Methanocaldococcus jannaschii |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Methanocaldococcus jannaschii | P81291 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| HisTrap HP column chromatography and Superdex 200 gel filtration | Methanocaldococcus jannaschii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| cis-homoaconitate | - |
Methanocaldococcus jannaschii | homoisocitrate + H2O | - |
? |  |
| cis-homoaconitate | - |
Methanocaldococcus jannaschii DSM 2661 | homoisocitrate + H2O | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | x-ray crystallography | Methanocaldococcus jannaschii |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Homoaconitase | - |
Methanocaldococcus jannaschii |
| leuC | large subunit | Methanocaldococcus jannaschii |
| MJ0499 | - |
Methanocaldococcus jannaschii |
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Release 2023.1 (January 2023)
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