Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
additional information | - |
complementation pair enzymes have higher molecular weights of 169000-185000 | Neurospora crassa |
88500 | - |
sucrose density gradient centrifugation, gel filtration | Neurospora crassa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-isopropylmaleate + H2O | Neurospora crassa | second of three reactions of leucine biosynthesis | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Neurospora crassa | - |
wild type and complementing Leu-2 mutants | - |
Purification (Comment) | Organism |
---|---|
- |
Neurospora crassa |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Neurospora crassa |
54000 | - |
- |
Neurospora crassa |
Storage Stability | Organism |
---|---|
-20°C, sodium phosphate buffer, pH 7.2, 6 M urea, several days | Neurospora crassa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-isopropylmaleate + H2O | second of three reactions of leucine biosynthesis | Neurospora crassa | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | amino acid analysis, protein contains hinge regions that are very sensitive to proteolytic attack | Neurospora crassa |
multimer | active as monomer, can aggregate to dimers or multimers in cell | Neurospora crassa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
34 | - |
enzyme assay at | Neurospora crassa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
enzyme assay at | Neurospora crassa |