Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | no requirement | Neurospora sp. | |
additional information | no requirement | Salmonella sp. |
General Stability | Organism |
---|---|
loss of activity after repeated freezing and thawing | Neurospora sp. |
loss of activity after repeated freezing and thawing | Salmonella sp. |
substrate stabilizes | Neurospora sp. |
substrate stabilizes | Salmonella sp. |
unstable in phosphate buffer at pH 7.0, 10% loss of activity per h at 0°C | Neurospora sp. |
unstable in phosphate buffer at pH 7.0, 10% loss of activity per h at 0°C | Salmonella sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | complete inhibition at 10 mM | Neurospora sp. | |
cysteine | complete inhibition at 0.004-0.02 mM | Neurospora sp. | |
dimethylmesaconate | - |
Neurospora sp. | |
Hg2+ | complete inhibition at 10 mM | Neurospora sp. | |
isopropylmalate | - |
Neurospora sp. | |
isopropylmalate | - |
Salmonella sp. | |
N-ethylmaleimide | 65% inhibition after preincubation in 1 mM | Neurospora sp. | |
N-ethylmaleimide | - |
Salmonella sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Ks for standard substrates | Neurospora sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | no requirement | Neurospora sp. | |
additional information | no requirement | Salmonella sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
90000 | - |
sucrose density gradient centrifugation, gel filtration | Neurospora sp. |
90000 | - |
sucrose density gradient centrifugation, gel filtration | Salmonella sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-isopropylmaleate + H2O | Neurospora sp. | high activities in leu-1 mutants which have accumulated great amounts of inducer | ? | - |
? | |
2-isopropylmaleate + H2O | Neurospora sp. | second of three reactions of leucine biosynthesis | ? | - |
? | |
2-isopropylmaleate + H2O | Salmonella sp. | second of three reactions of leucine biosynthesis | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Neurospora sp. | - |
- |
- |
Salmonella sp. | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Neurospora sp. |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
(2R,3S)-3-isopropylmalate = 2-isopropylmaleate + H2O | stoichiometry of reaction, enzyme also hydrates 2-isopropylmaleate to 3-hydroxy-4-methyl-3-carboxypentanoate, interconversion between the 2 isomers | Neurospora sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
12.3 | - |
- |
Neurospora sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-isopropylmaleate + H2O | - |
Neurospora sp. | 3-isopropylmalate | - |
? | |
2-isopropylmaleate + H2O | - |
Salmonella sp. | 3-isopropylmalate | - |
? | |
2-isopropylmaleate + H2O | high activities in leu-1 mutants which have accumulated great amounts of inducer | Neurospora sp. | ? | - |
? | |
2-isopropylmaleate + H2O | second of three reactions of leucine biosynthesis | Neurospora sp. | ? | - |
? | |
2-isopropylmaleate + H2O | second of three reactions of leucine biosynthesis | Salmonella sp. | ? | - |
? | |
3-isopropylmalate | - |
Neurospora sp. | 2-isopropylmaleate + H2O | - |
? | |
3-isopropylmalate | - |
Salmonella sp. | 2-isopropylmaleate + H2O | - |
? | |
additional information | citrate, aconitate, isocitrate are not substrates | Neurospora sp. | ? | - |
? | |
additional information | citrate, aconitate, isocitrate are not substrates | Salmonella sp. | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | trimer or tetramer of 2 different polypeptide subunits | Neurospora sp. |
trimer | trimer or tetramer of 2 different polypeptide subunits | Neurospora sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
95% loss of activity after 10 min | Neurospora sp. |
45 | - |
95% loss of activity after 10 min | Salmonella sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 8 | - |
Neurospora sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 8 | at pH 6.0, 20% of optimum activity, activity varies only about 20% between pH 7.0 and 8.0 | Neurospora sp. |
6 | 8 | at pH 6.0, 20% of optimum activity, activity varies only about 20% between pH 7.0 and 8.0 | Salmonella sp. |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 6.5 | unstable at pH 7.0 | Neurospora sp. |