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Literature summary for 4.2.1.32 extracted from

  • Reaney, S.K.; Begg, C.; Bungard, S.J.; Guest J.R.
    Identification of the L-tartrate dehydratase genes (ttdA and ttdB) of Escherichia coli and evolutioary relationship with the Class I fumarase genes (1993), J. Gen. Microbiol., 139, 1523-1530.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
overexpressed in DH5 alpha (pGS581) Escherichia coli

General Stability

General Stability Organism
cell extracts are prepared in extraction buffer and maintain at 0°C under N2 for more than 1 h before assaying for enzyme activity Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.76
-
(R,R)-Tartrate overexpressed strain DH5alpha(pGS581) Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
22641
-
2 * 22641 + 2 * 32589, DNA-derived values of TtdB and TtdA, quaternary structure of Escherichia coli K12 (W3110, wild-type) resembles that of the Pseudomonas putida enzyme Escherichia coli
32589
-
2 * 22641 + 2 * 32589, DNA-derived values of TtdB and TtdA, quaternary structure of Escherichia coli K12 (W3110, wild-type) resembles that of the Pseudomonas putida enzyme Escherichia coli
105000
-
gel filtration Escherichia coli
110500
-
DNA-derived values of TtdA and TtdB Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(R,R)-Tartrate Escherichia coli
-
?
-
?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
overexpressed in DH5alpha(pGS581)
-
Escherichia coli
-
K12 wild-type W3110
-

Oxidation Stability

Oxidation Stability Organism
oxygen-labile Escherichia coli

Storage Stability

Storage Stability Organism
-20°C, cell-extracts in air 90% loss of activity in less than 10 h Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(R,R)-Tartrate
-
Escherichia coli ?
-
?
(R,S)-Tartrate in the presence of glycerol and (R,S)-tartrate under anaerobic conditions Escherichia coli Oxaloacetate + H2O
-
?
L-(+)-Tartrate in the presence of glycerol (R,R)-tartrate can be used as reducible substrate for supporting anaerobic growth Escherichia coli Oxaloacetate + H2O
-
?

Subunits

Subunits Comment Organism
tetramer 2 * 22641 + 2 * 32589, DNA-derived values of TtdB and TtdA, quaternary structure of Escherichia coli K12 (W3110, wild-type) resembles that of the Pseudomonas putida enzyme Escherichia coli