Cloned (Comment) | Organism |
---|---|
overexpressed in DH5 alpha (pGS581) | Escherichia coli |
General Stability | Organism |
---|---|
cell extracts are prepared in extraction buffer and maintain at 0°C under N2 for more than 1 h before assaying for enzyme activity | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.76 | - |
(R,R)-Tartrate | overexpressed strain DH5alpha(pGS581) | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
22641 | - |
2 * 22641 + 2 * 32589, DNA-derived values of TtdB and TtdA, quaternary structure of Escherichia coli K12 (W3110, wild-type) resembles that of the Pseudomonas putida enzyme | Escherichia coli |
32589 | - |
2 * 22641 + 2 * 32589, DNA-derived values of TtdB and TtdA, quaternary structure of Escherichia coli K12 (W3110, wild-type) resembles that of the Pseudomonas putida enzyme | Escherichia coli |
105000 | - |
gel filtration | Escherichia coli |
110500 | - |
DNA-derived values of TtdA and TtdB | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R,R)-Tartrate | Escherichia coli | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
overexpressed in DH5alpha(pGS581) | - |
Escherichia coli | - |
K12 wild-type W3110 | - |
Oxidation Stability | Organism |
---|---|
oxygen-labile | Escherichia coli |
Storage Stability | Organism |
---|---|
-20°C, cell-extracts in air 90% loss of activity in less than 10 h | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R,R)-Tartrate | - |
Escherichia coli | ? | - |
? | |
(R,S)-Tartrate | in the presence of glycerol and (R,S)-tartrate under anaerobic conditions | Escherichia coli | Oxaloacetate + H2O | - |
? | |
L-(+)-Tartrate | in the presence of glycerol (R,R)-tartrate can be used as reducible substrate for supporting anaerobic growth | Escherichia coli | Oxaloacetate + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 2 * 22641 + 2 * 32589, DNA-derived values of TtdB and TtdA, quaternary structure of Escherichia coli K12 (W3110, wild-type) resembles that of the Pseudomonas putida enzyme | Escherichia coli |