Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 4.2.1.32 extracted from

  • Kelly, J.M.; Scopes, R.K.
    L-(+)-Tartrate dehydratase from Pseudomonas putida is an iron-sulphur enzyme (1986), FEBS Lett., 202, 274-276.
No PubMed abstract available
Search for more literature for 4.2.1.32

General Stability

General Stability Organism
manipulations on dye-ligand columns cause rapid irrecoverable loss of activity Pseudomonas putida
sucrose and/or succinate stabilize Pseudomonas putida

Inhibitors

Inhibitors Comment Organism Structure
Tartronic acid
-
 Pseudomonas putida

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ loss of activity in the absence of excess Fe2+ plus reducing agents Pseudomonas putida

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
23000
-
 2 * 23000 + 2 * 27000, SDS-PAGE Pseudomonas putida
27000
-
 2 * 23000 + 2 * 27000, SDS-PAGE Pseudomonas putida
100000
-
 gel filtration Pseudomonas putida

Organism

Organism UniProt Comment Textmining
Pseudomonas putida
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pseudomonas putida

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
78
-
-
 Pseudomonas putida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-(+)-Tartrate
-
 Pseudomonas putida Oxaloacetate + H2O
-
 ?

Subunits

Subunits Comment Organism
tetramer 2 * 23000 + 2 * 27000, SDS-PAGE Pseudomonas putida

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Pseudomonas putida