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Literature summary for 4.2.1.32 extracted from
- Tartaric acid metabolism (1965), J. Biol. Chem., 240, 2772-2777.
General Stability
| General Stability | Organism |
|---|---|
| 100 mM GSH plus 1mM Fe2+ optimal reactivation of inactive enzyme | Pseudomonas putida |
| crude cell-extract completely loses activity in open tubes in ice bath for 2-4 h, activity restored after incubation at 25°C for 30 min, extent of activation decreases with each succeeding cycle of inactivation and reactivation, loss of activity after treatment with EDTA or after dialysis | Pseudomonas putida |
| Cysteine 100 mM Cysteine plus 1 mM Fe2+ optimal reactivation of inactive enzyme | Pseudomonas putida |
| equimolar mixture of 50 mM GSH and 50 mM Cysteine plus 1 mM Fe2+ optimum reactivation of inactive enzyme | Pseudomonas putida |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| (R,S)-tartrate | competitive | Pseudomonas putida |  |
| CdCl2 | - |
Pseudomonas putida | |
| CoCl2 | - |
Pseudomonas putida | |
| CuCl2 | - |
Pseudomonas putida | |
| cysteine | 35% inhibition at 50 mM | Pseudomonas putida | |
| D-malic acid | K-salt of | Pseudomonas putida | |
| glutathione | 13% inhibition at 50 mM | Pseudomonas putida | |
| HgCl2 | - |
Pseudomonas putida | |
| L-Malic acid | K-salt of | Pseudomonas putida | |
| L-threonic acid | K-salt of | Pseudomonas putida | |
| Tartronic acid | K-salt of | Pseudomonas putida | |
| Urea | - |
Pseudomonas putida | |
| ZnCl2 | - |
Pseudomonas putida | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | reducing conditions: 2 KM-values/biphasic curve, presence of air: only one higher KM-value obtained | Pseudomonas putida | |
| 0.81 | - |
(R,R)-Tartrate | relatively anaerobic conditions | Pseudomonas putida | |
| 2.1 | - |
(R,R)-Tartrate | both, under anaerobic conditions and in the presence of air | Pseudomonas putida | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe2+ | addition of either GSH or cysteine plus Fe2+ reactivates inactive enzyme, optimum Fe2+ concentration 1 mM, Fe2+ only required for activation | Pseudomonas putida | |
| additional information | requirement for Fe2+ cannot be replaced by Mg2+, Ba2+, Mn2+, Co2+, Ni2+, Zn2+, Cu2+, Al3+, BO32-, MoO42- at concentrations of 0.01 mM and 1 mM | Pseudomonas putida |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 39000 | - |
sucrose density gradient centrifugation, inactive form. Aggregation of the four smaller units forms the active protein | Pseudomonas putida |
| 145000 | - |
sucrose density gradient centrifugation, active form. Enzyme exists as an aggregate of smaller protein or proteins, the latter possessing no enzyme activity | Pseudomonas putida |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (R,R)-Tartrate | Pseudomonas putida | - |
? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas putida | - |
- |
- |
Oxidation Stability
| Oxidation Stability | Organism |
|---|---|
| reactivation of the inactive enzyme is seriously inhibited by exposure to air | Pseudomonas putida |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Pseudomonas putida |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (R,R)-Tartrate | - |
Pseudomonas putida | ? | - |
? | |
| L-(+)-Tartrate | - |
Pseudomonas putida | Oxaloacetate + H2O | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 39000 sucrose density gradient centrifugation, inactive form, activation by aggregation of 4 monomeres | Pseudomonas putida |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Pseudomonas putida |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
- |
Pseudomonas putida |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7.6 | 9.4 | 50% of maximal activity at pH 7.6 and at pH 9.4 | Pseudomonas putida |
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