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Literature summary for 4.2.1.30 extracted from

  • Zhang, G.; Xu, X.; Li, C.; Ma, B.
    Cloning, expression and reactivating characterization of glycerol dehydratase reactivation factor from Klebsiella pneumoniae XJPD-Li (2009), World J. Microbiol. Biotechnol., 25, 1947-1953.
No PubMed abstract available

Activating Compound

Activating Compound Comment Organism Structure
ATP
-
Klebsiella pneumoniae
coenzyme B12
-
Klebsiella pneumoniae
DhaF glycerol dehydratase reactivation factor Klebsiella pneumoniae
DhaG glycerol dehydratase reactivation factor Klebsiella pneumoniae

Cloned(Commentary)

Cloned (Comment) Organism
pET-28a(+)-dhaBCEE expressed in Escherichia coli BL21 (DE3) Klebsiella pneumoniae

Inhibitors

Inhibitors Comment Organism Structure
glycerol GDHt can be quickly reactivated by glycerol dehydratase reactivation factor to 98% in the presence of ATP, Mg2+ and coenzyme B12. In the first 10 min of the reactivation reaction, the average reactivation rate is 0.18 micromol/min for glycerol-inactivated GDHt Klebsiella pneumoniae
O2 GDHt can be quickly reactivated by glycerol dehydratase reactivation factor in the presence of ATP, Mg2+ and coenzyme B12. In the first 10 min of the reactivation reaction, the average reactivation rate is 0.12 micromol/min for O2-inactivated GDHt Klebsiella pneumoniae

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
Klebsiella pneumoniae

Organism

Organism UniProt Comment Textmining
Klebsiella pneumoniae
-
-
-
Klebsiella pneumoniae XJPD-Li
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,2-propanediol
-
Klebsiella pneumoniae propionaldehyde + H2O
-
?
1,2-propanediol
-
Klebsiella pneumoniae XJPD-Li propionaldehyde + H2O
-
?
glycerol
-
Klebsiella pneumoniae 3-hydroxypropanal + H2O
-
?
glycerol
-
Klebsiella pneumoniae XJPD-Li 3-hydroxypropanal + H2O
-
?

Synonyms

Synonyms Comment Organism
GDHt
-
Klebsiella pneumoniae
glycerol dehydratase
-
Klebsiella pneumoniae